睫状神经营养因子和生长相关蛋白-43在兔眶内慢性视神经损伤中的表达  

Expression of CNTF and GAP-43 in chronic damage of intraorbital optic nerve in rabbit

作　　者：刘秀明[1,2] 魏锐利[1] 蔡季平[1] 

机构地区：[1]上海第二军医大学附属长征医院眼科,研究生200003 [2]江苏省淮安市第一人民医院眼科,223300

出　　处：《眼科》2010年第2期135-138,共4页Ophthalmology in China

摘　　要：目的建立眶内视神经慢性受压损伤动物模型,并观察睫状神经营养因子(CNTF)和生长相关蛋白-43(GAP-43)在视神经中的表达。设计实验研究。研究对象新西兰大白兔36只。方法将动物分为6组,即A组(无压迫组):只放置球囊而不充盈造影剂;B组(2w):逐量注入造影剂压迫2周;C组(2w+2w):压迫2周后解除压迫2周;D组(4w):注入造影剂压迫4周;E组(4w+4w):压迫4周解除压迫4周;F组(8w):压迫8周。用眼眶内植入气囊并定时注入造影剂的方法 ,模拟眼眶肿瘤缓慢生长对眶内视神经慢性压迫,建立视神经慢性损伤动物模型;用RT-PCR方法检测在眶内视神经慢性损伤后不同阶段(造模后2、4、8周)CNTF和GAP-43的基因表达。主要指标CNTF和GAP-43的表达。结果眼底像和组织病理检查显示,随着压迫时间的延长,眼底、视网膜和视神经都出现病理改变并逐渐加重。空白及无压迫组低表达CNTF和GAP-43,压迫2周后即可见表达上调(P<0.01),随着压迫时间延长,两种基因均见表达逐渐增高(P<0.01)。结论成功建立兔眶内视神经慢性损伤动物模型,并观察到随着压迫时间的延长,CNTF和GAP-43在视神经中的表达量逐渐增多,提示神经细胞被外来损伤诱导,开始进行自身修复。Objective To construct a chronic intraorbital optic nerve compression damage model in rabbits and to observe the expression of CNTF and GAP-43 in the optic nerve. Design Experimental study. Participants New Zealand white rabbits. Methods The animals were divided into six groups： group A （balloon implanted but without contrast medium injection）, group B （compress for 2 weeks by injecting increase volume of contrast medium）, group C （compress for 2 weeks and decompress for another 2 weeks）, group D （compress for 4 weeks by injecting increase volume of contrast medium）, group E （compress for 4 weeks and decompress for another 4 weeks）, group F （compress for 8 weeks）. A balloon was implanted into the orbit of New Zealand rabbits, in which a contrast medium was filled at designated time points to simulate chronic a tumor compression on the intraorbital optic nerve. RT-PCR was performed for gene assays at different phases （at 2, 4 and 8 weeks after modeling） of chronic intraorbital optic nerve damage. Main Outcome Measures The expression of ciliary neurotrophic factor （CNTF）and growth associated protein 43 （GAP-43）. Results Fundus photography and histopathological examinations revealed the pathologic changes of the retina and optic nerve became more severe along with the compressing time. CNTF and GAP-43 expression was low in the blank and non compression groups, and up-regulated after 2w compression （P〈0.01）; with the compression time prolonging, expression of the two genes increased gradually （P〈0.01）. Conclusions A chronic intraorbital optic nerve compression damage model was constructed successfully, whereby we found that with the compression time prolonging, CNTF and GAP-43 expression increased gradually 2 weeks after damage. （Ophthalmol CHN, 2010, 19： 135-138）

关 键 词：视神经 慢性损伤 动物模型 基因表达 

分 类 号：R774.6[医药卫生—眼科]