利用^(18)F-FDGPET-CT显像监测裸鼠鼻咽癌移植瘤早期放疗疗效的研究  

Early monitoring of radiotherapeutic effects of nasopharyngeal carcinoma xenografts in nude mice using ^(18)F-FDG PET-CT imaging

作　　者：袁建伟[1] 冯彦林[1] 冼伟均[1] 贺小红[1] 袁白虹[1] 叶秋莲[2] 

机构地区：[1]中山大学附属佛山医院核医学科,广东佛山528000 [2]中山大学附属佛山医院儿科,广东佛山528000

出　　处：《癌症》2010年第4期409-414,共6页Chinese Journal of Cancer

基　　金：吴阶平医学基金(320.6750.06113);广东省医学科学技术研究基金(A2006657)~~

摘　　要：背景与目的:早期监测和判断鼻咽癌放疗疗效,并提供个体化治疗是提高鼻咽癌患者生存率的重要因素之一。本研究拟探讨18F-FDG PET-CT显像在裸鼠鼻咽癌移植瘤早期放疗疗效评价中的价值,为进一步临床研究提供依据。方法:所有10只裸鼠鼻咽癌移植瘤动物模型于放疗前均先行18F-FDG PET-CT显像,并于分组前对其中7只裸鼠分别行早期和3h延迟PET-CT显像。而后将10只裸鼠鼻咽癌移植瘤动物模型随机分成对照组和放疗组。对照组的5只动物在显像完毕后即刻处死并取瘤体标本行病理学检查;放疗组的5只动物在接受12Gy放疗后的第2天、第4天、第6天再次行18F-FDG PET-CT显像,显像结束后即刻取瘤体标本行病理学检查。应用ROI技术测定瘤体T/NT比值和放疗前、后的瘤体积。结果:早期显像和延迟显像T/NT比值分别为1.806±0.532、1.777±0.597,两者之间差异无统计学意义(P>0.05)。放疗组在放疗前与放疗后第2天、第4天、第6天的T/NT比值分别为1.735±0.466、1.818±0.396、1.096±0.101和0.604±0.108;瘤体积分别为(1.48±0.27)cm3、(1.57±0.31)cm3、(1.59±0.31)cm3和(1.60±0.29)cm3。放疗后第6天与其他时间点瘤体T/NT比值差异有统计学意义(P<0.05),且放疗6天后瘤体死亡细胞比例为(93.00±7.42)%。结论:18F-FDG PET-CT显像在裸鼠鼻咽癌移植瘤早期放疗疗效评价中具有重要价值,且放疗后第6天可能是合适的时间选择点。放疗前18F-FDG PET-CT延迟显像的T/NT比值测定对鼻咽癌诊断可能没有意义。Background and Objective：Monitoring the therapeutic effects of radiotherapy for nasopharyngeal carcinoma （NPC） is critical to providing individualized treatment. This in-vivo study was initially designed to evaluate the therapeutic effect of radiotherapy using ^18F-fluorodeoxyglucose positron emission tomography with computed tomography （^18F-FDG PET-CT） imaging. Methods：^18F-FDG PET-CT imaging was performed on all of the 10 nude mice bearing NPC xenografts before radiotherapy,and early-phase and delayed-phase PET-CT images were performed on 7 of the 10 mice. All mice were randomly divided into either a control group or a radiotherapy group. The 5 mice in the control group were immediately killed after the imaging and pathology were performed. After receiving radiotherapy of 12 Gy,5 animals in the radiotherapy group were given ^18F-FDG PET-CT imaging on days 2,4,and 6,and then were killed for pathologic evaluation. Regions of interest （ROI） technology was used to measure the tumor target/non-target （T/NT） ratio and the volume of the tumors. Results：The average T/NT ratios of early-and delayed-phase imaging were 1.806± 0.532 and 1.777± 0.597,respectively,with no significance （ P〉0.05）. For the radiotherapy group,the average T/NT ratios for ^18F-FDG PET-CT before radiotherapy,and on days 2,4,and 6 after radiotherapy,were 1.735± 0.466,1.818± 0.396,1.096± 0.101,and 0.604± 0.108,respectively,and the tumor volumes were （1.48± 0.27） cm^3,（1.57± 0.31） cm^3,（1.59± 0.31） cm^3,and （1.60± 0.29） cm^3,respectively. The average T/NT ratios of day 6 after radiotherapy and the other time points were significantly different （ P〈0.05）. The average death ratio of the tumor cells was （93.00± 7.42）% after 6 days of post-radiotherapy.Conclusions：^18F-FDG PET-CT imaging can be used for the early assessment of radiotherapeutic effect of NPC in vivo. Day 6 after radiotherapy may be an appropriate time point for the imaging. However,the T/NT ratio measurement of delayed-pha

关 键 词：裸鼠 鼻咽癌 移植瘤 体层摄影术 发射型计算机 放射治疗 

分 类 号：R739.63[医药卫生—肿瘤]