患溃烂症的眼斑拟石首鱼分离的灿烂弧菌的鉴定  

Identification of a Vibrio splendidus strain isolated from ill Sciaenops ocellatus

作　　者：马爱敏[1,2] 闫茂仓[1,2] 胡利华[1,2] 谢起浪[1,2] 陈少波[1,2] 单乐州[1,2] 邵鑫斌[1,2] 

机构地区：[1]浙江省海洋水产养殖研究所,温州325005 [2]浙江省近岸水域生物资源开发与保护重点实验室,温州325005

出　　处：《微生物学报》2010年第4期542-547,共6页Acta Microbiologica Sinica

基　　金：浙江省科技厅科研院所专项公益技术攻关项目(2007F10009)~~

摘　　要：【目的】从患溃烂症的眼斑拟石首鱼分离到优势菌株M-1,并进一步对该菌的系统发育学进行了分析。【方法】采用形态学、生理生化鉴定,结合16SrRNA和HSP60基因序列分析。【结果】16SrRNA基因同源性分析,菌株M-1与灿烂弧菌(AJ874361、AY046955)聚为一群;HSP60基因(groES)序列分析表明M-1与弧菌(EU653883、AY837440)聚为一个分支。【结论】菌株M-1属于灿烂弧菌(Vibrio splendidus)。Objective We isolated bacterial strain named M-1 from ill Sciaenops ocellatus with ulceration,and further analyzed the phylogenetics of the bacterial strain.MethodsWe characterized the strain on the basis of its morphology, physiology,and sequence comparison of 16S rRNA and HSP60 genes.ResultsStrain M-1 was identified as Vibrio splendidus.16SrRNA gene of strain M-1 and Vibrio splendidus（AJ874361 ） had nearest kinship,and the analysis of HSP60 gene sequence indicated that strain M-1 and Vibrio（EU653883,AY837440） located in the same phylogenetic tree.ConclusionThe results suggested that strain M-1 was a member of Vibrio splendidus.

关 键 词：M-1菌株 致病性 16SRRNA基因 HSP60基因 灿烂弧菌 

分 类 号：S941[农业科学—水产养殖]