云南白药对富小板血浆促兔骨髓基质干细胞增殖分化影响的研究  

Influence of YunnanBaiyao on the stimulating effects of platelet-rich plasma on proliferation and differentiation of rabbits bone marrow stromal stem cells

作　　者：佘睿[1] 张明珠[1] 税艳青[1] 储雯[1] 雷雅燕[1] 

机构地区：[1]昆明医学院附属口腔医院口腔内科,云南昆明650021

出　　处：《牙体牙髓牙周病学杂志》2010年第3期127-130,共4页Chinese Journal of Conservative Dentistry

基　　金：云南省教育厅科研基金资助课题(07C10582)

摘　　要：目的:研究云南白药对富血小板血浆(Platelet-Rich Plasma,PRP)促兔骨髓基质干细胞增殖(rabbits Bone Marrow Stromal Stem Cells,rBMSCs)和分化的影响。方法:取4月龄新西兰大耳白兔骨髓,用贴壁筛选法并结合传代纯化骨髓基质干细胞,并采用流式细胞术对其鉴定;观察第1代～第8代细胞的增殖情况,绘制生长曲线;采用Landesberg法分别制备PRP;分别用不同浓度云南白药配合PRP对rBMSCs培养;用四唑盐比色法(MTT)和酶动力学方法检测云南白药对PRP促rBMSCs增殖和分化的影响。结果:与对照组相比,60μg/mL(0.53±0.03)、65μg/mL(0.56±0.05)组对rBMSCs的增殖作用有显著性差异(P<0.05);65μg/mL(1.21±0.08)、70μg/mL(1.28±0.05)、75μg/mL(1.24±0.06)组云南白药可显著增强rBMSCs的ALP活性(P<0.05)。结论:云南白药对PRP促rBMSCs的增殖和分化有促进作用。AIM：To investigate whether YunnanBaiyao affects the stimulating effects of platelet-rich plasma （PRP） on the proliferation and differentiation of rabbit bone marrow stromal stem cells （rBMSCs）. METHODS： rBMSCs were isolated from 4-month-old New Zealand rabbit bone marrow and selected by the adherent method. Flow cytometry was used to confirm the identification of rBMSCs. The 3rd passage rBMSCs were plated at 5×104 /well in 96 well plates in L-DMEM. Cells were treated with PRP plus various concentrations of Yunnanbaiyao. Cells without treatment and with PRP treatment only served as controls. The proliferation of rBMSCs was examined by MTT method and the alkaline phosphatase （ALP） activity was determined by enzyme assay on day 3, 5 and 7. RESULTS： At concentration of 60 μg/mL and 65 μg/mL Yunnanbaiyao showed statistically significant enhancing effects on PRP- stimulated rBMSCs proliferation at all time points （P0.05）. At concentrations of 60, 65, 70 and 75 μg/mL Yunnanbaiyao greatly enhanced the stimulating effects of PRP on rBMSCs ALP levels on day 5 and day 7 （P0.05）. However, no significant difference was observed between the PRP and PRP＋ Yunnanbaiyao group in ALP activity on day 3 （P0.05）. CONCLUSION： YunnanBaiyao can promote the stimulatory effects of PRP on the proliferation and differentiation of rBMSCs.

关 键 词：骨髓基质干细胞 富血小板血浆 云南白药 增殖 分化 

分 类 号：R780.2[医药卫生—口腔医学]