环氧合酶和诱导型一氧化氮合酶在人牙周病牙龈组织中的表达变化  

Expression of COX-2 and iNOS in gingival tissues of patients with chronic periodontitis

作　　者：祁红延[1] 陈莉娅[1] 张志瑞[1] 

机构地区：[1]兰州大学口腔医学院,甘肃兰州730000

出　　处：《牙体牙髓牙周病学杂志》2010年第3期139-142,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:研究人牙周病牙龈组织环氧合酶(COX-2)和诱导型一氧化氮合酶(iNOS)的表达变化。方法:取牙周炎病人牙龈组织15例,牙龈炎病人牙龈组织15例、正畸或第三磨牙拔除时的正常龈组织14例,制作切片进行COX-2和iNOS免疫组织化学染色,观察比较牙龈组织中COX-2、iNOS的表达变化。结果:①COX-2在正常牙龈组织中几乎不表达,在牙龈炎、牙周炎龈组织中均有阳性表达。其中牙周炎组最高,与正常组相比有显著性差异(P<0.05),但与牙龈炎组相差不显著(P>0.05),牙龈炎组与正常组相比亦无显著性差异(P>0.05);②iNOS在正常牙龈组织中几乎不表达,在牙龈炎和牙周炎龈组织中均有阳性表达。其中正常组最低,与牙龈炎组和牙周炎组相比均有显著性差异(P<0.05),而牙龈炎组与牙周炎组相比则无显著性差异(P>0.05)。结论:牙周病牙龈组织中COX-2、iNOS表达升高,可能成为牙周病时组织破坏、牙槽骨吸收的影响因素之一。AIM：To evaluate the cyclooxgenase-2 （COX-2） and inducible nitric oxide synthase （iNOS） expression in periodontal tissues. METHODS：Fifteen patients with chronic periodotitis （CP）,15 patients with gingivitis （G） and 15 health controls （H） were included in this study. Gingival tissues were surgically excised. COX-2 and iNOS expression were detected with immunohistochemistry staining. RESULTS：COX-2 and iNOS were not detected in healthy gingival tissues. Positive staining of COX-2 and very strong positive staining of iNOS were detected in the ginvival tissues of CP and G groups. The expression of COX-2 in CP group was significantly higher than that in H group （P〈0.05）. No significant difference of COX-2 expression was found between the CP and G group, and also between the G and H group （P〉0.05）. The expression of iNOS in CP and G group was significantly higher than that in H group （P〈0.05）. No significant difference of iNOS expression was found between the CP and G groups （P〉0.05）. CONCLUSION： COX-2 and iNOS expression ware elevated in the inflamed gingival tissues of gingivitis and chronic periodontitis. COX-2 and iNOS may play important roles in the destruction of periodontal tissues.

关 键 词：环氧合酶 诱导型一氧化氮合成酶 牙周炎 免疫组化技术 

分 类 号：R781.4[医药卫生—口腔医学]