北京油鸡与AA肉鸡脂肪组织差异表达基因的研究  

Screening and Identification of Differentially Expressed Genes in Beijing Fatty Chicken and Broiler Adipose Tissue

作　　者：苗向阳[1] 冯浩咏[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国生物工程杂志》2010年第3期40-45,共6页China Biotechnology

基　　金：国家"863"计划(2008AA10Z140);"十一五"国家科技支撑计划(2006BDA13B08)资助项目

摘　　要：运用mRNA差异显示技术对AA肉鸡和北京油鸡脂肪组织基因的差异表达进行研究,从分子遗传学角度分析导致两品种脂肪组织差异表达的原因,对了解性状形成的遗传基础和调控机理是十分必要的。通过反Northern杂交验证共筛选出脂肪组织差异表达基因10条,经与GenBank数据库进行相似性比对,XF1与已知基因有较高同源性,该基因是人类cDNA全长开放阅读框(ORF)的一段;XF2、YF1、YF2及YF4经与nr数据库进行同源性比对,均可找到同源性较高的基因,但功能未知;XF4与克隆人类胎盘CL0BA010ZF08基因的一段cDNA序列同源性为83%;YF3与预测原鸡MLL5(LOC417712)基因有一定的同源性,目前尚无功能报道;XF5和YF5与原鸡高迁移率族蛋白(HMGN3)有较高同源性;XF3在nr库中未找到同源序列,确定为新发现的EST,提交数据库获得GenBank登录号(Accession number:EU594549)。为进一步研究北京油鸡与AA肉鸡脂肪组织差异基因的功能与脂肪发育的关系奠定基础。Differential display reverse transcription PCR（DDRT-PCR） was used to identify differential expression genes in Beijing fatty chicken and Arbor Acres Broiler（AA） adipose tissue.A total of 10 ESTs were found using DDRT-PCR and reverse northern dot blot,all of 10 ESTs were compared with nucleotide sequences deposited in the nr database and Gallus database.XF2、YF1、YF2 and YF4 had their highly similar nucleotide sequences in nucleotide databases but with unknown functions.XF4 is similar to full-length cDNA clone CL0BA010ZF08 of Placenta of Homo sapiens,the identity is 83%;YF3 was found highly similar to MLL5 （LOC417712）,but the function is unknow;XF5 and YF5 were highly similar to HMGN3;XF3 had no significant similarity with existing genes or ESTs and was regarded as new EST.The new EST was submitted to GenBank（Accession number：EU594549）.The lays a foundation for further study on the mechanism of differential gene expression in Beijing fatty and AA adipose tissue.

关 键 词：北京油鸡 AA肉鸡 差异显示 脂肪组织 

分 类 号：Q786[生物学—分子生物学]