不同外源性途径干预血管新生对脑缺血模型大鼠神经功能缺失修复的影响  

Different exogenous interventions in angiogenesis to repair neurological deficits in cerebral ischemia rats

作　　者：齐志国[1] 梁红梅 朱晓峰[3] 谢鹏[1] 

机构地区：[1]重庆医科大学第一附属医院神经内科,重庆400016 [2]黑龙江省富锦市中心医院超声科,黑龙江富锦156100 [3]佳木斯大学,黑龙江佳木斯154002

出　　处：《第三军医大学学报》2010年第8期778-782,共5页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(973计划;2009CB008300)~~

摘　　要：目的探讨脑缺血区血管新生和神经功能缺失修复的关系以及选择较理想的外源性干预途径增加脑缺血区血管新生。方法选择240只SD大鼠分为假手术组、缺血对照组、缺血加VEGF腹腔注射组、缺血加血管内皮祖细胞移植组和缺血加血管内皮因子单克隆抗体组,在1、3、7、14、30、60 d观察各组脑缺血及周边区的血管密度和大鼠的电生理指标(体感诱发电位和运动诱发电位)及行为学(感觉损伤移交实验、轮转跑步机运动功能测定和mNSS改良神经功能评分)。结果缺血加VEGF腹腔注射组、缺血加血管内皮祖细胞移植组的新生血管密度除1 d外其他各时间点均高于缺血对照组(P<0.05),缺血加血管内皮因子单克隆抗体组在后5个时间点新生血管密度小于缺血对照组(P<0.05)。缺血加VEGF腹腔注射组、缺血加血管内皮祖细胞移植组和缺血加血管内皮因子单克隆抗体组在7 d后体感诱发电位和运动诱发电位与缺血对照组有统计学差异(P<0.05)。与缺血对照组相比,缺血加VEGF腹腔注射组、缺血加血管内皮祖细胞移植组和缺血加血管内皮因子单克隆抗体组在3 d后行为学评分有统计学差异(P<0.05)。结论通过外源性干预脑缺血模型大鼠缺血区血管新生,可以影响神经功能缺失的修复;且单次移植血管内皮祖细胞与VEGF多次注射用药可以达到相同的效果。repair through exogenous interventions,and investigate optimal intervention routes for angiogenesis in cerebral ischemia rats.Methods Totally 240 SD rats were randomly divided into sham operation group,ischemic control group,ischemia and vascular endothelial growth factor（VEGF） group [1 653.3 ng/（g·d）,i.p.,for 10 d],ischemia and endothelial progenitor cell transplantation（EPCs） group（2×1010/L,10 μl）,ischemia and anti-VEGF monoclonal antibody group [3 mg/（kg·d）,i.p.,for 10 d].Cerebral microvessel density,somatosensory evoked potentials（SEP） and motor evoked potentials（MEP） and behavior were observed in 1,3,7,14,30 and 60 d after injury.Results New blood vessel density of ischemia and VEGF group,ischemia and EPCs group were significantly higher than ischemic control group in all time points besides 1 d after injury（P〈0.05）.On the contrary,ischemia and anti-VEGF group was lower than ischemic control group in posterior 5 time points（P〈0.05）.SEP and MEP of ischemic control group and ischemia and VEGF group,ischemia and EPCs group,ischemic and anti-VEGF group had statistically significant differences from ischemic control group after 7 d（P〈0.05）.Behavior was significantly different among all groups after 3 d（P〈0.05）.Conclusion Exogenous intervention in cerebral ischemia rats with ischemic angiogenesis can affect repair of neurological deficits.And VEGF therapy through multiple injections and a single transplanted endothelial progenitor cells can receive similar results.

关 键 词：脑缺血 血管新生 神经功能 

分 类 号：R363.27[医药卫生—病理学] R364.33[医药卫生—基础医学]