葛根总黄酮对不同急性髓系白血病细胞株增殖抑制的影响  

Inhibitory Effect of Flavonoids of Puerarin on Proliferation of Different Human Acute Myeloid Leukemia Cell Lines In Vitro

作　　者：邵化敏[1] 唐宇宏[1] 姜鹏君[2] 朱红青[1] 章亚成[2] 季建敏[2] 季鸥[1] 沈群[2] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]南京中医药大学第一附属医院血液科,江苏南京210029

出　　处：《中国实验血液学杂志》2010年第2期296-299,共4页Journal of Experimental Hematology

基　　金：江苏省卫生厅"科教兴卫工程"医学重点人才课题(编号RC2007068);江苏省"六大人才高峰"第五批高层次人才项目(2008年)

摘　　要：为了探讨葛根总黄酮对急性髓系白血病(acute myeloid leukemia,AML)的作用及其可能的机制,观察不同浓度葛根总黄酮体外对4种AML细胞株Kasumi-1、HL-60、NB4和U937的增殖抑制影响和细胞周期的变化。应用MTT法检测细胞株细胞的增殖抑制作用,用流式细胞术检测细胞周期的变化。结果表明:一定浓度葛根总黄酮对4种AML细胞株增殖抑制作用差异显著,抑制强度依次为NB4细胞>Kasumi-1细胞>U937细胞>HL-60细胞;葛根总黄酮不同程度地改变NB4、Kasumi-1、U937及HL-60细胞周期进程,使G1期细胞比例下降,S期细胞比例增高,出现亚二倍体峰,呈现浓度依赖性。结论:葛根总黄酮体外对不同AML细胞株具有选择性增殖抑制作用,以对NB4细胞株的影响最为显著。This study was aimed to investigate the inhibitory effect of flavonoids of puerarin （PR） in different concentrations on proliferation of 4 kinds of acute myeloid leukemia （AML） cell lines （ Kasumi-1, HL-60, NB4 and U937 ）, and to explore its possible mechanism. The MTT method was used to detected the inhibitory effect of PR on proliferation of AML cell lines. The flow cytometry was adopted to determine the change of cell cycle in vitro. The results showed that a certain concentration of PR could inhibit the proliferation of these 4 cell lines effectively in time-and dose-dependent manners, and the intensity of inhibition on 4 kinds of AML cell lines was from high to low as follows ： NB4 〉 Kasumi-1 〉 U937 〉 HL-60. Meanwhile, PR could also change cycle process, cell proportion in G1/G0 phase decreased, cells in S phase increased and Sub-diploid peak also appeared. It is concluded that PR can selectively inhibit the proliferation of 4 AML cell lines and block cell cycle process, especially for NB4 cells.

关 键 词：葛根总黄酮 AML细胞株 增殖抑制 细胞周期 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]