多发性骨髓瘤病人骨髓细胞中Cmtm5基因异常低表达  

Abnormally Lower Expression of Cmtm5 Gene in Bone Marrow Cells from Patients with Multiple Myeloma

作　　者：牛继红[1] 鲍立[1] 张瑶[1] 李金兰[1] 李玲娣[1] 谢敏[1] 秦亚溱[1] 赖悦云[1] 江倩[1] 史惠琳[1] 刘艳荣[1] 江滨[1] 陈珊珊[1] 黄晓军[1] 阮国瑞[1] 

机构地区：[1]北京大学人民医院、北京大学血液病研究所,北京100044

出　　处：《中国实验血液学杂志》2010年第2期363-367,共5页Journal of Experimental Hematology

基　　金：中国国家自然科学基金项目(编号30670894);北京大学人民医院研究与发展基金资助(编号RDC2009-39)

摘　　要：本研究检测cmtm5(CKLF-like MARVEL transmembrane domain containing member5)基因在多发性骨髓瘤(MM)骨髓细胞中的表达水平,并探索其与临床特征的关系。应用实时定量逆转录-聚合酶链反应(real-time quantitative RT-PCR,RQ-RT-PCR)技术检测MM患者骨髓细胞、MM细胞系及正常人骨髓细胞cmtm5基因的表达。以cmtm5拷贝数与abl拷贝数的比值表示cmtm5基因的相对表达水平。结果表明,51例初治及难治复发MM患者骨髓细胞cmtm5表达量(0.047±0.062)显著低于20例正常人骨髓细胞cmtm5表达量(0.255±0.333,p<0.01)。ISSⅢ期患者组cmtm5基因表达量(0.034±0.034)显著低于ISSⅠ期患者组的表达量(0.103±0.109,p<0.01)。两种人多发性骨髓瘤细胞系RPMI8226及CZ1的cmtm5基因表达量(为0.014±0.009、0.004±0.006)均低于正常人骨髓细胞。15例治疗有效患者的cmtm5基因表达量与治疗前及复发时相比显著升高(0.020±0.005;0.227±0.038,p<0.01)。cmtm5基因的表达量与骨髓浆细胞比例呈负相关(r=-0.307,p<0.05),而与性别、年龄、血红蛋白水平、M蛋白量、免疫球蛋白分型、IgH是否阳性等没有明显的相关性。结论:多发性骨髓瘤患者骨髓细胞cmtm5基因表达水平降低,且与ISS分期有关,与骨髓浆细胞数呈负相关,此结果说明cmtm5基因在MM的发病机制中发挥一定作用。This study was aimed to detect the expression level of cmtm 5 （CKLF-like MARVEL transmembrane domain containing member 5 ） gene in the bone marrow cells from patients with multiple myeloma （ MM）, and to investigate the correlation between the expression level of cmtm5 and various clinical characteristics. Real-time quantitative reverse transcription polymerase chain reaction （RQ-RT-PCR） was used to measure the expression levels of cmtm5 gene in the bone marrow cells collected from MM patients, and the MM cell lines, namely, RPMI8226 and CZ1 cells. The normal donor marrow specimens were used as the reference. The ratio of cmtm5 copy number to abl （ Abelson murine leukemia viral oncogene homolog） gene copy number was used for indicating the expression level. The results showed that the expression level of cmtm5 gene was significantly down-regulated in bone marrow cells of 51 untreated or relapsed/refractory MM patient as compared to those of normal donor marrow cells （0.047 ±0.062 for the untreated or relapsed/refractory MM patients versus O. 255 ± 0. 333 for the normal, p 〈 0.01 ）. According to the International Staging System （ISS）, the cmtm5 expression level in marrow cells of patients in ISS Ⅲ stage was significantly lower than that in patients in ISS I stage （0.034 ±0.034 for the ISS Ⅲ stage versus 0. 103 ±0. 109 for ISS I stage, p 〈 0.01 ）. Similarly, lower expression levels of cmtm5 gene were also found in two human MM cell lines （0.014 ±0. 009 for RPMI8226 cells and 0. 004 ± 0. 006 for CZ1 cells）. After the MM patients were effectively treated, their expression levels of cmtm5 gene significantly increased （0. 020 ± 0.005 for the untreated patients versus O. 227 ± 0. 038 for the effectively treated patients ,p 〈 0.01 ）. A significant negative correlation was observed between the expression level of cmtm5 gene and the number of bone marrow plasma cells （ r = - 0. 307, p 〈0. 05 ）. However, the correlation was not found between the expression level of

关 键 词：cmtm5 多发性骨髓瘤 骨髓细胞 

分 类 号：R733.3[医药卫生—肿瘤]