HSP90表达与人多发性骨髓瘤细胞迁移力的相关性研究  

Correlation of HSP90 mRNA Expression with Migration Ability of Human Multiple Myeloma Cells

作　　者：黄灵娟[1] 马艳萍[1] 杨薏蓉[1] 杨林花[1] 

机构地区：[1]山西医科大学第二临床医院血液内科,山西太原030001

出　　处：《中国实验血液学杂志》2010年第2期372-375,共4页Journal of Experimental Hematology

基　　金：山西省留学人员科研资助项目;编号20081099

摘　　要：本研究探讨热休克蛋白90(HSP90)与人多发性骨髓瘤细胞迁移力的相关性。采用反转录聚合酶链反应(RT-PCR)检测经终浓度为50、100、150和200nmol/L的蛋白酶体抑制剂硼替佐米作用于人多发性骨髓瘤细胞株U266 4小时后细胞中HSP90的mRNA的表达情况。应用Transwell小室迁移实验检测经上述相同浓度硼替佐米作用4小时后U266细胞迁移力的变化。结果表明:随着硼替佐米药物浓度的增加,U266细胞中HSP90α的mRNA表达量逐渐增加(p<0.05),而HSP90β的mRNA表达量虽然总体上有差别(p<0.05),但增加趋势不很明显。随着药物浓度的增加U266细胞迁移力逐渐减弱(p<0.05)。结论:HSP90表达与人多发性骨髓瘤细胞迁移力存在相关性。This study was aimed to investigate the correlation of heat shock protein 90 （HSP90） expression with migration ability of human multiple myeloma cells. The HSP90 mRNA expression and migration change of human multiple myeloma cell line （U266） were detected by RT-PCR and Transwell chamber respectively after treatment of U266 cells with final concentration 50, 100, 150,200 nmol/L of bortezomib （proteosome inhibitor） for 4 hours. The results indicated that along with the increasing of bortezomib concentration, the expression level of HSP90a mRNA in U266 cells was enhanced, while no obvious increase of HSP9013 mRNA expression was observed in spite of statistical difference as a whole （p 〈 0.05 ）, but with the increaseing of drug concentration in cells, their migration ability gradually decreased （p 〈 0.05）. It is concluded that the corelation of HSP90 expression with migration ability of human multiple myeloma cells exists.

关 键 词：多发性骨髓瘤细胞株 U266细胞 HSP90 硼替佐米 细胞迁移 

分 类 号：R733.3[医药卫生—肿瘤]