Livin在非霍奇金淋巴瘤中表达的临床意义  

Clinical Significance of Livin Expression in Non-Hodgkin′s Lymphoma

作　　者：高钧明[1] 王欣[1] 刘小倩[1] 葛学玲[1] 丁梅[1] 王丽黎[1] 

机构地区：[1]山东大学附属省立医院血液科,山东济南250021

出　　处：《中国实验血液学杂志》2010年第2期385-390,共6页Journal of Experimental Hematology

基　　金：山东省科技发展计划项目(编号2007GG10002008);山东省自然科学基金(编号Y2007C053)

摘　　要：本研究旨在探讨凋亡抑制因子Livin在非霍奇金淋巴瘤组织中的表达及其临床意义。应用免疫组织化学法检测Livin蛋白在30例非霍奇金淋巴瘤(non-Hodgkin′s lymphoma,NHL)患者及11例淋巴结反应性增生(reac-tive hyperplasia of lymph node)患者淋巴结中的表达,并应用real-time PCR对其中20例非霍奇金淋巴瘤、10例淋巴结反应性增生及4例正常人的淋巴结组织中Livin mRNA表达水平进行检测,分析livin蛋白和mRNA表达与NHL患者临床表现及其特征的相关性。结果表明,NHL患者淋巴结组织中Livin mRNA表达水平与正常淋巴结组织以及淋巴结反应性增生组织比较均显著增高(RQ中位数分别为12.4vs0.34和12.4vs0.61)(p<0.01)。30例非霍奇金淋巴瘤中有16例Livin蛋白表达阳性,阳性率为53.3%;11例淋巴结反应性增生患者仅1例表达阳性,阳性率为9.1%;而且观察到Livin蛋白主要在细胞浆中表达,在胞核内极少见有表达。研究还显示,无论是LivinmRNA表达还是蛋白表达均与NHL的临床分期(p=0.023;p=0.009)、B症状(p=0.015;p=0.026)、血β2微球蛋白(β2-MG,p=0.031;p=0.012)及血清乳酸脱氢酶(LDH,p=0.037;p=0.007)密切相关,而两者的表达与年龄、性别及分型无明显相关性(p>0.05)。结论:Livin mRNA及蛋白在NHL患者中表达增高,并与临床多项指标存在相关性,因此Livin表达水平对评价患者临床分期及预后可能具有重要的指导意义;对Livin在NHL中的作用机制的深入探讨将有助于揭示NHL的发生、发展及治疗的机理,并有望成为NHL未来治疗的重要突破点。This study was aimed to explore chnical significance of Livin mRNA and protein expressions in non- Hodgldng lymphoma（NHL）. The immunohistochemistry was used to determine the expression of Livin protein in lymph nodes of 30 patients with NHL and 11 patients with reactive hyperplasia of lymph node. The real-time PCR was performed to detect the expression levels of Livin mRNA in 20 patients with NHL, 10 patints with reactive hyperplasia of lymph node and 4 normal person lymph nodes. The correlation of Livin mRNA and protein expressions with NHL clinical features were analyzed. The results showed that the expression of Livin InRNA was statistically higher in NHL samples than in normal lymph nodes and reactive hyperplastic lymph nodes （ 12.4 vs 0. 34, 12. 4 vs 0. 61, median） （p 〈 0.05 ）, while there was no statistical difference between normal and reactive hyperplastic lymph nodes （p 〉 0.05 ）. Livin protein expression was exhibited to be positive in 16 of 30 cases of NHL with a positive rate of 53.3% and only 1 in reactive hyperplastic lymph nodes with a positive rate of 9. 1%. In addition, Livin protein almost appeared in the cytoplasm of cells, seldom in nucleus. The expressions of both Livin mRNA and protein were positively correlated with clinical stages of NHL （p = 0. 023;p =0.009） ,B symptoms （p =0. 015;p =0.026）, blood β2-microglobulin（ β2-MG, p =0.031 ;p =0.012） and the serum level of lactate dehydrogenase （LDH ,p = 0. 037 ;p = 0.007 ）, but the expressions of Livin mRNA and protein had no significant correlation with age, sex and typing. It is concluded that the Livin mRNA and protein highly express in NHL patients and correlate with many chnical features, such as stage of NHL, B symptom, β2-MG and LDH, therefore, the Livin may play a role in the prognosis of NHL patients. The further study on inhibitiory effect of Livin expression will promote the illustration of NHL pathogenesis and contribute to the treatment and prognosis of NHL.

关 键 词：LIVIN 非霍奇金淋巴瘤 淋巴结反应性增生 

分 类 号：R733[医药卫生—肿瘤]