氩氦冷冻治疗后的荷C6胶质瘤大鼠的磁共振成像和病理研究  

Experimental study of magnetic resonance imaging and pathologies in rats with C6 gliomas after cryotherapy

作　　者：李明[1] 黄凡衡[2] 胡丽娟[3] 周媛[3] 姜晓丹[1] 常海刚[1] 卢凤飞[1] 陈强[1] 张世忠[1] 

机构地区：[1]南方医科大学珠江医院神经外科 广东神经外科研究所 广东省脑功能修复重点实验室,广州510282 [2]南方医科大学珠江医院放射科 广东神经外科研究所 广东省脑功能修复重点实验室,广州510282 [3]南方医科大学珠江医院肿瘤科 广东神经外科研究所 广东省脑功能修复重点实验室,广州510282

出　　处：《中华神经医学杂志》2010年第4期354-357,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨荷C6胶质瘤大鼠经氩氦刀治疗后的影像学和病理学的变化。方法将51只Wistar大鼠背部皮下植入体外扩增的C6胶质瘤细胞，制作荷C6胶质瘤的动物模型，模型制作成功后按照随机数字表法分为假手术组（暴露肿瘤后只插入冷冻刀头不启动氩氦冷冻系统，n=18），外科手术切除组（将肿瘤完整切除，不进行冷冻操作，n=15）和氩氦冷冻组（暴露肿瘤后启动氩氦冷冻系统，n=18）。观察各组大鼠治疗前后MRI影像变化，应用Tunel检测细胞凋亡情况。结果外科手术成功切除胶质瘤。氩氦冷冻组MRI显示T1WI信号较术前增强，T2WI信号较术前减低假手术组治疗后MRI无明显变化。氩氦冷冻组肿瘤组织Tunel染色可见大量凋亡细胞，外科手术切除组及假手术组可见少量散在的凋亡细胞。结论经氩氦刀治疗胶质瘤MRI提示肿瘤细胞变性坏死．病理学提示可以介导细胞凋亡，有望成为脑胶质瘤的一种有效的辅助治疗手段。Objective To explore the changes of imaging and pathologies in rats with bearing C6 gliomas after cryotherapy. Methods Animal models with subcutaneously transplanting C6 gliomas in the Wistar rates were established. Fifty-one rat models were randomized into sham-operated group （n=18）, resection group （n=15） and cryotherapy group （n=18）. The tumors in the sham-operated group were exposed only and the tumors in the resection group were resected without the help of freezing system; the tumors in the cryothempy group were performed argon-helium cryoablation. The changes of morphology and imaging were observed. TUNEL staining was employed to observe the cell apoptosis in the tumors. Results The tumors in the resection group were removed successfully by microsurgical resection. Cryotherapy group showed significantly enhanced T 1WI signal and weak T2WI signal after the treatment as compared with that before the treatment; no obvious difference of the MRI data was found between before and after the treatment in the sham-operated group. The cell apoptosis of the tumor was conformed in the cryotherapy group by Tunel staining; while only a few apoptotic cells were noted in the resection and sham-operated groups. Conclusion Cryotherapy can not only destroy the tumor cells but also induce the cell apoptosis, suggesting its great potential in the clinical management of gliomas.

关 键 词：神经胶质瘤 氩氦刀 磁共振成像 病理学 

分 类 号：R739.4[医药卫生—肿瘤]