不同组织来源人嗅神经鞘细胞生物学与免疫学特性的对比研究  

Biological and immunological properties of human olfactory ensheathing cells derived from different tissues： a comparative study

作　　者：吴燕峰[1] 任明亮[2] 黄晓明[3] 王潇娉[1] 梁新军[2] 杨睿[2] 黄霖[2] 唐勇[2] 王鹏[2] 沈慧勇[2] 

机构地区：[1]中山大学孙逸仙纪念医院生物治疗中心,广州510120 [2]中山大学孙逸仙纪念医院骨外科,广州510120 [3]中山大学孙逸仙纪念医院耳鼻喉科,广州510120

出　　处：《中华神经医学杂志》2010年第4期358-362,共5页Chinese Journal of Neuromedicine

基　　金：基金项目：广东省自然科学基金（7001603）;广州市科技计划项目（2008A1-E4011-9）;教育部博士点（20070558256）

摘　　要：目的比较成人嗅球与嗅黏膜来源的嗅神经鞘细胞（OECs）的生物学与免疫学特性，探讨后者取代前者行细胞或组织移植治疗的可行性。方法分别分离、培养、纯化两种来源的OECs．应用MTT法测定细胞活力，结合对比骨髓间充质干细胞（BMSCs）的免疫特性，应用免疫细胞化学染色、定量技术、流式细胞术、双向混合淋巴细胞反应等方法来比较分析不同组织来源的人OECs的生物学与免疫学特性。结果两种OECs开始分化时间相同，细胞形态基本一致；生长曲线与活力曲线均走行一致，曲线大部分重合；免疫细胞化学p75NTR染色两者均呈阳性；流式分析两种OECs均不表达CD34与B7-1（CD80）、B7-2（CD86）、HLA—DR、CD40、CD40L等移植免疫标志；嗅球源性嗅神经鞘细胞组双向混合淋巴细胞反应抑制率（51．18％±6．01％）与嗅黏膜源性嗅神经鞘细胞组（51．00％±5．87％）、BMSCs组（52．79％±3．12％）比较，差异均无统计学意义（P〉0．05）。结论成人嗅球与嗅黏膜来源的OECs在生物学与免疫学特性方面基本一致．可考虑使用来源更为便捷的成人嗅黏膜源性OECs代替嗅球源性OECs行细胞或组织移植治疗。Objective To compare the biological and immunological properties of human olfactory ensheathing cells （OECs） derived from human adult olfactory bulb and olfactory mucosa, and discuss the feasibility that the latter takes the place of the former in the utilization of cell or tissue transplantation therapy. Methods Human OECs derived from different tissues were isolated, cultured and purified; MTT assay was employed to observe the cell viability. With the characteristics of bone mesenchymal stem cells （BMSCs） as comparison, immunocytochemical staining, quantitative techniques, flow cytometry and two-way mixed lymphocytes reaction method were used to compare and analyze the biological and immunological properties of OECs derived from human adult olfactory bulb and olfactory mucosa. Results The 20ECs derived from human adult olfactory bulb and olfactory mucosa started to differentiate at the same time and had the same cell morphology basically. The growth curve was in accordance with the vitality curve and had many coincident points; positive immunohistochemistry P75sax staining was found in both 2 kinds of OECs. Flow cytometry showed no expressions of CD34, B7-1 （CD80）, B7-2（CD86）, HLA-DR, CD40 and CD40L. No significant differences on the rate of two-way mixed lymphocytes reaction were found among HAOBOECs group, HAOMOECs group and BMSCs group （51.18%±6.01%, 51.00%±5.87%and51.00%±5.87%, respectively, P〉0.05） Conclusion Human adult olfactory bulb-derived and olfactory mucosa-derived OECs are basically the same biological and immunological properties. Human adult olfactory mucosa-derived OECs, a more convenient source, can take the place of human adult olfactory bulb-derived OECs in the utilization of cell or tissue transplantation therapy.

关 键 词：嗅鞘细胞 嗅黏膜 嗅球 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]