大黄素对人肺腺癌A549细胞体外增殖凋亡及VEGF和TNF-α分泌的影响  

Effects of Emodin on Cell Proliferation, Apoptosis and VEGF/TNF-α Secretion of Human Lung Adenocarcinoma A549 Cells

作　　者：夏启松[1] 刘静维[1] 孙仁宇[1] 修瑞娟[1] 

机构地区：[1]中国医学科学院北京协和医学院微循环研究所,北京100005

出　　处：《肿瘤防治研究》2010年第4期387-391,共5页Cancer Research on Prevention and Treatment

摘　　要：目的为大黄素抗肺腺癌的临床应用提供理论依据。方法MTT实验检测大黄素不同浓度梯度(5μmol/L,10μmol/L,20μmol/L,40μmol/L,80μmol/L)和时间梯度(12,24,48和72h)处理后A549细胞的增殖活力;Annexin V-FITC/PI双染流式细胞术和Hoechst 33258染色法检测大黄素作用A549细胞后引起的细胞凋亡程度;应用酶联免疫吸附分析(ELISA)实验检测大黄素各处理组培养上清液中所含的VEGF、TNF-α的浓度。结果大黄素体外可浓度和时间依赖性地抑制人肺腺癌A549细胞的增殖活力及促进细胞凋亡,抑制A549细胞VEGF的表达和分泌,促进TNF-α的表达和分泌。结论大黄素具有抗增殖、促凋亡、抗血管生成等多种潜能,具有良好的抗人非小细胞肺癌特别是肺腺癌的临床应用前景。Objective To study the effects of emodin on cell proliferation, apoptosis and secretion of vascular endothelial growth factor-A （VEGF） and tumor necrosis factor-α （TNF-α） of human lung adenocarcinoma A549 cells in vitro.Methods MTT assay was applied to determine the cell proliferation of A549 cells after treated with emodin at different concentrations （5, 10, 20, 40, 80 μmol/L） for different time durations （12, 24, 48 and 72 h）. The apoptotic A549 cells were double-color stained with annexin V-FITC and propidium iodide （PI） and then measured with flow cytometry （FCM）; The apoptotic A549 cells were also stained by Hoechst 33258 and examined under fluorescence microscope. The culture medium supernatants of A549 cells treated by emodin were collected and concentrations of VEGF and TNF-α were analyzed using enzyme-linked immunosorbent assay （ELISA）.Results Emodin significantly inhibited cell proliferation and promoted apoptotic rate of human lung adenocarcinoma A549 cells, and reduced VEGF expression and secretion but enhanced TNF-α expression and secretion in concentration-and time-dependent manners.Conclusion Emodin has potentials of anti-proliferation, pro-apoptosis and anti-angiogenesis in human lung adenocarcinoma A549 cells, so that emodin could be developed as a new antitumor agent for non-small cell lung cancer （NSCLC） therapy.

关 键 词：大黄素 肺腺癌 细胞增殖/凋亡 血管内皮生长因子 肿瘤坏死因子-Α 

分 类 号：R734.2[医药卫生—肿瘤]