大网膜乳斑在胃癌腹膜转移中的作用  

The function of omental milky spot in gastric cancer peritoneal mestastasis

作　　者：曹亮[1] 胡祥[1] 孙希同[1] 张怡[2] 安伟德[1] 

机构地区：[1]大连医科大学附属第一医院普外科,116011 [2]大连医科大学美容医学院

出　　处：《中华实验外科杂志》2010年第4期433-434,I0001,I0002,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察大网膜乳斑在胃癌腹膜转移中的作用。方法胃癌细胞株MFC，经Dil标记后，以每只小鼠1×10^4个Dil-MFCs注射到72只615小鼠腹腔内，不同时间取大网膜，观察Dil—MFCs在乳斑和非乳斑区的变化。结果Dil-MFCs注入小鼠腹腔12h后，Dil—MFCs开始大量出现在乳斑区；48h，在巨噬细胞杀伤作用下，仅有少量细胞存活；72h后，乳斑区可见增殖细胞团，形成微转移增殖灶。在非乳斑区，48h可见散在少量的Dil—MFCs，72h后散在Dil—MFCs增多，未发现增殖细胞团。腹腔注射14d，乳斑区形成以乳斑为中心的团状转移灶，而非乳斑区则形成单细胞转移。结论胃癌细胞在腹膜转移的早期阶段选择性地侵犯乳斑，乳斑转移灶是腹膜进一步转移的基础。Objective To study the function of omental milky spot in gastric cancer peritoneal mestastasis. Methods 1 ×10^4 Dil labeled MFC cells were injected intraperitoneally （72 mice） , and the animals were killed at different time points after intraperitoneal injection. The omenta was excised for immunohistochemistry. Results At the 12th h post injection, gastric cancer cells were particularly concentrated in milky spots. After 48 b, number of gastric cancer cells was declined in milky spot areas for the reason of the killing function of macrophages. After 72 h, number of DiI-MFCs was further decreased in milky spot areas, and proliferating tumor cells were observed in milky spots and formed micrometastasis. In contrast, sporadic gastric cancer cells in non-milky spot areas were found at the 48th h after intraperitoneal injection. After 72 h, number of Dil-MFCs in milky spots was increased. Two weeks after intraperitoneal injection, milky spot area was occupied totally by the proliferating gastric cancer cells, and gastric cancer cells formed cell cluster type metastasis. But the proliferating cancer cells weren＇ t observed in non-milky spot areas at the same stage, and cancer cells formed single cell typc metastasis. Conclusion Cancer cells selectively infiltrate into the milky spots at the early stages of peritoneal cancer dissemination ; the cell cluster type metastasis in milky spots becomes a main cell source of further peritoneal metastasis.

关 键 词：胃癌 腹膜 转移 乳斑 

分 类 号：R735.2[医药卫生—肿瘤]