人膀胱移行细胞癌上皮间质转化与胸腺素β4的关系  

Correlation between thymosin β4 and epithelial-mesenchymal transition in human bladder transitional cell carcinoma

作　　者：朱朝辉[1] 曾甫清[1] 王智宇[1] 吕磊[1] 万锋[1] 邢诗安[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022 [2]华中科技大学同济医学院附属协和医院中心实验室,武汉430022

出　　处：《中华实验外科杂志》2010年第4期497-500,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30872561）

摘　　要：目的探讨人胸腺素β（Tβ）与膀胱移行细胞癌（BTCC）上皮-间质转化的相关性，以及与临床病理特征之间的关系。方法选用膀胱移行细胞癌手术切除标本60例，每例标本均选用癌组织中心、癌旁组织及其远端正常黏膜对照。采用定量逆转录-聚合酶链反应（RT—PCR）、Westernblot和免疫组织化学技术检测切除标本中的Tβ4、整合素连接激酶（ILK）、E—cadherin和β-catenin的表达，并分析与各临床病理因素之间的关系。结果Tβ4在BTCC组织中的表达明显高于正常黏膜（91．7％比15．0％，P〈0．05），其在深层浸润组中的表达高于浅层浸润组（97．4％比81．0％，P〈0．05）；ILK在BTCC组织中的表达明显高于正常黏膜（80．0％比18．3％，P〈0．05）；β-catenin在BTCC组织中的表达高于正常黏膜（76．7％比26．7％，P〈0．05）；E—cadherin在BTCC组织中的表达明显低于正常黏膜（30．0％比90．0％，P〈0．05）；Tβ4与E—cadherin表达呈负相关，与ILK、β-catenin表达呈正相关。结论胸腺素β与膀胱移行上皮癌分化和转移有关，可能通过调控上皮-间质转化从而在膀胱移行上皮癌的侵袭转移过程中发挥作用。Objective To investigate the relationship between human thymosin β4（Tβ4） and epithelial-mesenchymal transition （EMT） in bladder transitional cell carcinoma （BTCC）, explore the association with clinicopathologic features. Methods 60 BTCC specimens from surgically resected were selected. For each specimen, the cancerous tissue, peri-cancer tissue and its remote normal mucosa were analyzed and compared. The expression of Tβ4, integrin-linked kinese （ILK）, E-cadherin and β-catenin proteins in resected cancer tissues were detected by using real-time reverse transcription-polymerase chain reaction （ RTPCR）, Western blotting and immunohistochemistry, and compared with clinicopathologic data. Results （ 1 ） The expression rate of Tβ4 was significantly higher in BTCC tissues than in normal tissues （91.7% vs 15.0% ,P 〈0. 05） ,and the expression rate of Tβ4 in deeply infiltrating group was significantly higher than that in superficially infiltrating group （97.4% vs 81% ,P 〈 0. 05 ） ;the level of ILK was significantly higher in BTCC than in normal tissues（80.0% vs 18. 3% ,P 〈0. 05） ;the expression rate of β-catenin was higher in BTCC tissues than in normal tissues （ 76. 7% vs 26. 7%, P 〈 0.05 ） ; the expression of E-cadherin in BTCC tissues were significantly lowered in comparison with those in normal bladder epithelium （30. 0% vs 90. 0%, P 〈 0. 05 ） ; （2） Tβ4 expression was inversely correlated to E-cadherin, but positively to ILK and β-catenin. Conclusion Thymosin β4 is related to the differentiation and metastasis of bladder transitional cell carcinoma, and may induce EMT to promote BTCC metastasis.

关 键 词：上皮-间质转化 膀胱移行细胞癌 胸腺素Β4 

分 类 号：R737.14[医药卫生—肿瘤]