p53、c-erbB-2癌基因蛋白表达与胃癌生物学行为的关系  

Relationship between the expression of p53 and cerbB2 proteins and biologic behaviors of human gastric carcinoma

作　　者：卢敏华[1,2] 杜卫东[1,2] 魏伟[1,2] 王春华 

机构地区：[1]江苏省徐州市第一人民医院病理科 [2]中国科学院上海生理研究所

出　　处：《临床与实验病理学杂志》1998年第1期20-22,I004,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：探讨ｐ５３、ｃ－ｅｒｂＢ－２表达与胃癌生物学行为的关系。方法：利用微波免疫组化方法研究７０例胃癌及２０例胃癌前病变ｐ５３、ｃ－ｅｒｂＢ－２蛋白的表达。结果：正常胃粘膜和肠化生上皮ｐ５３及ｃ－ｅｒｂＢ－２均阴性，中、重度异型增生及胃癌组织中ｐ５３阳性率分别为１５．０％和５１．４％。ｃ－ｅｒｂＢ－２阳性率分别为１０．０％和４０．０％。ｐ５３与胃癌组织学类型、组织发生及分化程度等有关（Ｐ＜０．０５），而ｃ－ｅｒｂＢ－２则与胃癌分化程度、胃癌浸润深度等有关（Ｐ＜０．０５）。在低分化腺癌癌巢边缘部，硬癌内呈浸润性生长的细胞以及脉管内转移性癌栓等处，除了癌细胞核内ｐ５３表达较强外，胞浆也呈弥漫性染色，表明胞浆阳性的癌细胞具有更强的侵袭性。在非浸润进展组或随患者生存期的延长，胃癌组织内ｐ５３及ｃ－ｅｒｂＢ－２的阳性率有下降的趋向。结论：ｐ５３可作为临床筛选胃癌、癌前期病变以及进行早期诊断的有用指标，ｐ５３与ｃ－ｅｒｂＢ－２一起可用于判断胃癌预后。Purpose To study the relationship between the expression of p53 and cerbB2 proteins and biologic behaviors of human gastric carcinoma. Methods Expressions of oncogene protein p53 and cerbB2 in 70 cases of human gastric carcinomas and 20 cases of precancerous lesions (moderatesevere dysplasia) were investegated with microwaveimmunohistochemistry. Results Expressions of p53 and cerbB2 proteins were negative in normal gastric mucosa and intestinal metaplasia. The incidences of p53 protein positivity in moderatesevere dysplasia and gastric carcinomas were 150% and 514% respectively and those of cerbB2 protein were 100% and 400% respectively. The expression of p53 protein in human gastric carcinoma tissues was related to histological patterns, histogenesis and differentiated degree of cancer (P<005), While the expression of cerbB2 protein was related to differentiated degree and the infiltration depth of cancer (P<005). The overexpression of p53 protein in nuclei and cytoplasms of gastric carcinoma was much more dominant around the nests of low differentiated adenocarcinoma, in the infiltrating cancer cells in scirrhous carcinoma and the cancer emboli in infiltrated vessels. The cancer cells with p53 positive cytoplasmic staining possessed a st ronger ability of invasion. Besides, the expressions of p53 and cerbB2 proteins showed a declining tendency in both the nonmetastasis and the more than 5year survival groups. Conclusions p53 protein, together with cerbB2, might be useful in screening precancerous lesions and determining early diagnosis of gastric carcinoma as well as in evaluating prognosis and observing patient's progression.

关 键 词：胃肿瘤 免疫组织化学 P53基因 C-ERBB-2基因 

分 类 号：R735.202[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]