食管癌变过程中p53、c-myc、bcl-2表达及其与细胞凋亡的关系  

Relationship between the expression of p53, cmyc, bcl2 and the apoptosis in esophageal carcinogenesis

作　　者：王立东[1,2,3] 杨万才[1,2,3] 周琦[1,2,3] 贾云英 

机构地区：[1]河南医科大学癌症研究室 [2]新乡医学院病理教研室 [3]河南省濮阳市人民医院

出　　处：《临床与实验病理学杂志》1998年第2期106-108,I017,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：河南省杰出青年科学基金;国家教委优秀青年教师基金

摘　　要：目的：探讨食管癌变过程中肿瘤抑制基因ｐ５３和癌基因ｃ－ｍｙｃ、ｂｃｌ－２的变化及其与细胞凋亡的关系。方法：采用免疫组化法（ＡＢＣ）检测２７９例食管粘膜活检组织中ｐ５３、ｃ－ｍｙｃ、ｂｃｌ－２的表达以及细胞凋亡的变化。结果：从食管正常上皮到基底细胞增生、间变和癌，ｐ５３、ｃ－ｍｙｃ、ｂｃｌ－２免疫阳性表达率及细胞凋亡发生率和细胞凋亡指数（ＡＩ）均呈升高趋势，而且在同一阶段病变，ｐ５３和ｃ－ｍｙｃ阳性表达时凋亡指数高于其阴性表达，而ｂｃｌ－２阳性表达时凋亡指数低于其阴性表达。结论：在食管癌变过程中可能有多种肿瘤抑制基因和癌基因参与，细胞凋亡在食管癌变过程中可能有重要的生物学意义。Purpose To investigate the relationship between the changes of tumor suppressor gene p53 and oncogenes cmyc, bcl2 and apoptosis in esophageal carcinogenesis. Methods Immunohistochemical method was carried out to detect the expression of p53, cmyc, bcl2 for 279 patients from esophageal endoscopic mucosa biopsies, and quantitive analysis for apoptosis was made. Results Immunoreactivity of p53, cmyc and bcl2, and occurrence of apoptosis and apoptotic index (AI)(the number of apoptotic cells and apoptotic bodies/mm2) showed increase from esophageal normal epithelia to basal cell hyperplasia and dysplasia and squamous cell carcinoma, and in the similar lesions p53 and cmyc positive lesions showed slightly higher AI than p53 and cmyc negative lesions. However, bcl2 positive lesions had lower AI than bcl2 negative lesions. Conclusions Multiple genes may involve in and apoptosis may have an important biological significance in esophageal carcinogenesis.

关 键 词：食管肿瘤 细胞凋亡 P53基因 C-MYC基因 

分 类 号：R735.102[医药卫生—肿瘤]