丹参酮ⅡA诱导HL-60细胞凋亡  

Apoptosis of human leukemic HL-60 cells induced by Tanshinone ⅡA

作　　者：黄韧敏[1] 袁淑兰[1] 宋毅[1] 黄光琦[1] 

机构地区：[1]华西医科大学肿瘤研究所

出　　处：《癌症》1998年第3期164-166,I000,共4页Chinese Journal of Cancer

摘　　要：目的：在以往对丹参酮ＩＡ诱导分化和抗癌作用研究的基础上，进一步研究丹参酮ＩＡ诱导ＨＬ－６０细胞凋亡，以探讨其抗癌作用机理。方法：应用体外细胞培养技术，以１～１０μｇ／ｍｌ丹参酮ＩＡ作用于培养的ＨＬ－６０细胞５天后，进行光镜、电镜、琼脂糖凝胶电泳及流式细胞仪的观察和分析。结果：丹参酮ⅡＡ作用后，ＨＬ－６０细胞的生长受到明显抑制，其半数抑制浓度ＩＣ５０约为１０μｇ／ｍｌ光镜、电镜观察到细胞呈现凋亡特征，琼脂糖凝胶电泳显示细胞ＤＮＡ呈梯状降解，流式细胞仪分析表明，１０μｇ／ｍｌ丹参酮ⅡＡ作用后，ＨＬ－６０细胞凋亡率达２８．８％，细胞被阻止于Ｇ０／Ｇ１期，Ｓ期细胞明显减少（Ｐ＜０．０１），其ｂｃｌ－２基因的表达显著降低，而ｐ５３基因的表达增强（Ｐ＜０．０１）。结论：丹参酮ⅡＡ可诱导ＨＬ－６０细胞凋亡，这可能为丹参酮ⅡＡ抗癌抑癌的重要机理之一，诱导分化与诱导凋亡之间的关系有待进一步探讨。Purpose: Based on the tumor cell differentiation and antitumor effects induced by Tanshinone, the apoptosis of HL-60 cells induced by Tanshinone IIA was studied in order to find out the mechanism of the antitumor effect of Tanshinone IIA. Methods: By techniques of cell culture in vitro, HL-60 cells were treated by Tanshinone IIA in 1～10 μg/ml concentrations for 5 days, then observed and analysed by light microscopy, electronic microscopy, agarose gel electrophoresis and Flow-Cytometry (FCM). Results: The growth of HL-60 cells was inhibited evidently after treatment by Tanshinone IIA, the 50% inhibitory concentration (IC50) was about 10 μg/ml. The cells showed characters of apoptosis observed by light and electronic microscopy, Agarose gel electrophoresis of DNA showd change of “ladder” pattern. The ratio of apoptosis is 28.8% when treated with 10 μg/ml Tanshinone IIA, and the cell was inhibited in G0/G1 phase, the cells in S phase decreased (P<0.01). The expression of bc1-2 gene protein decreased obviously and p53 gene protein increasd when analysed by FCM (P<0.01). Conclusions: The results once more confirmed that Tanshinone IIA could induce the apoptosis of HL-60 cells, which may be one of the important mechanisms of Tanshinone IIA's antitumor effects. Further work about the relationship between cell differentiation and apoptosis induced is needed.

关 键 词：丹参酮ⅡA 细胞凋亡 白血病细胞 HL-60 

分 类 号：R733.7[医药卫生—肿瘤]