EB病毒基因在喉鳞状细胞癌中的表达  

Expression of Epstein-Barr virus gene in squamous cell carcinoma of the larynx

作　　者：刘克拉[1] 宗永生 

机构地区：[1]中山医科大学

出　　处：《癌症》1998年第1期10-12,F004,共4页Chinese Journal of Cancer

摘　　要：目的：ＥＢ病毒感染与喉鳞状细胞的发生发展是否有关迄今未明。本文目的在于了解ＥＢ病毒基因在喉鳞状细胞癌组织中的表达情况，探讨各种ＥＢ病毒基因产物在喉鳞状细胞癌发生发展中的生物学意义。方法：采用原位杂交和免疫组化技术，检测２９例不同分化类型喉鳞状细胞癌组织中ＥＢ病毒编码的小ＲＮＡ（ＥＢＥＲｓ）、潜伏感染膜蛋白（ＬＭＰ１）、ＥＢ病毒核抗原（ＥＢＮＡ－１）、溶解感染立即早期基因编码蛋白ＺＥＢＲＡ、早期基因编码蛋白ＥＡ－Ｄ、晚期基因编码蛋白ＶＣＡ和ＭＡ。结果：各检测指标在未分化癌、低分化和中分化鳞癌均有一定比例的阳性，但在２例高分化鳞癌均为阴性。发现ＥＢ病毒潜伏性和溶解性感染基因产物表达分别随着癌分化程度增高而减弱和增强。７例低分化和１例中分化鳞癌ＬＭＰ－１除胞膜和胞浆阳性外，胞核也呈明显阳性。２例低分化和１例中分化鳞癌中有少量癌细胞ＺＥＢＲＡ阳性。结论：部分喉鳞状细胞癌尤其是未分化和低分化鳞癌的发生发展与ＥＢ病毒感染密切相关，提示癌细胞的分化程度与ＥＢ病毒基因表达的差异有关。Purpose:The expression of Epstein-Barr virus gene in the squamous carcinoma of larynx is not determined so far.The aim of this paper is to study the expression of Epstein-Barr virus gene products and their probable biological function in carcinogenesis of laryngeal squamous carcinoma.Method:Twenty-nine cases of laryngeal squamous cell carcinoma with various grade of differentiation were studied on the expression of Epstein-Barr virus early RNAs(EBERs)、LMP-1、EBNA-1、ZEBRA、EA-D、VCA and MA by using in situ hybridization and immunohistochemistry.Results:The results showed that these EBV gene products could be detected with different rates in undifferentiated、poorly-differentiated and moderately differentiated squamous cell carcinoma except for 2 cases of well-differentiated squamous cell carcinoma.It was found that the undifferentiated carcinoma was strongly positive for EBV latent gene products but weakly positive for lytic gene products, while the status of latent and lytic gene expression in moderately and some poorly-differentiated squamous cell carcinoma was contrary to that of the undifferentiated carcinoma. Seven cases of poorly-differentiated and 1 case of moderately differentiated squamous cell carcinoma wer srongly positive for LMP-1 in nucleus as well as in cell membrane and cytoplasm.It was also noted that there were a small number of ZEBRA positive cells in 2 poorly-differentiated and 1 moderately differentiated squamous cell carcinoma.Conlusions:The results confirmed a consistent association between EBV infection and some laryngeal squamous cell carcinoma especially for undifferentiated and poorly-differentiated carcinoma.It was suggested that the expression of EBV genes were related to the status of differentiation in host cells.The biological significance of EBV gene expression in the laryngeal squamous cell carcinoma was discussed.　　

关 键 词：鳞状细胞癌 喉肿瘤 E-B病毒 基因表达 

分 类 号：R739.650.2[医药卫生—肿瘤] R373.11[医药卫生—临床医学]