硒化壳聚糖对NB4细胞血管内皮生长因子表达及分泌的影响  

Effect of selenium chitosan on the expression and secretion of VEGF in NB4 cells

作　　者：邓守恒[1] 曹凤军[1] 李芳[1] 邓守平[2] 陈萍[1] 

机构地区：[1]郧阳医学院附属人民医院肿瘤中心,十堰442000 [2]郧阳医学院附属人民医院消化内科

出　　处：《陕西医学杂志》2010年第4期387-389,共3页Shaanxi Medical Journal

基　　金：湖北省教育厅基金项目(B200624004);十堰市科学技术局基金(十科通[2006]18号)项目

摘　　要：目的:探讨硒化壳聚糖对白血病细胞株NB4细胞VEGF mRNA表达及VEGF蛋白分泌的变化。方法:应用RT-PCR法和ELISA法研究硒化壳聚糖对NB4细胞VEGF mRNA表达及VEGF蛋白分泌的影响。结果:NB4细胞中有VEGF mRNA的表达及VEGF蛋白的分泌,硒化壳聚糖在体外能下调VEGF mRNA的表达和VEGF蛋白的分泌,呈剂量依赖关系。结论:硒化壳聚糖除可通过诱导NB4细胞分化或诱导其凋亡外,还可通过抑制促血管新生达到发挥抗白血病的效应。Objective：TO investigate the expression of VEGF mRNA and secretion of VEGF protein in NB4 cells treated by selenium chitosan . Methods：Semi-quantitative RT-PCR and ELISA were used to study the expression of VEGF mRNA and secretion of VEGF protein in NB4 cell treated by selenium chitosan . Results：VEGF was expressed in NB4 cell. The expression of VEGF mRNA and secretion of VEGF protein could be down regulated by selenium chitosan in a dose dependent manner. Conclusion：Besides inducing apoptosis and promoting differention of Leukemia cells,selenium chitosan were exerted its anti-Leukemia effects by reducing angiogenesis via reduction of angiogenic reaction stimulating signals.

关 键 词：壳多糖 硒化合物 白血病 粒细胞 急性/免疫学 血管生成因子/分析 内 皮血管 @NB4 

分 类 号：R733.71[医药卫生—肿瘤]