树实验肝癌发生过程中IGF-Ⅱ、HBxAg、p21和p53蛋白的表达  

Expressions of IGF Ⅱ, HBxAg, p21 and p53 in development of experimental hepatocellular carcinomas in tree shrews

作　　者：苏建家 严瑞琪[1,2] 覃国忠 黄定瑞[1,2] 杨春 

机构地区：[1]广西肿瘤研究所病理室 [2]中山医科大学肿瘤研究所

出　　处：《临床与实验病理学杂志》1998年第5期484-487,I064,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：探讨ＨＢＶ与ＡＦＢ１协同致肝癌作用机制。方法：用免疫组化观察５３只树鼠句肝及肝癌组织ＩＧＦ－Ⅱ、ＨＢｘＡｇ、ｐ２１及ｐ５３蛋白表达。结果：接受ＨＢＶ和ＡＦＢ１的树鼠句肝癌诱发率和ＩＧＦ－Ⅱ、ＨＢｘＡｇ、ｐ２１检出率分别为５２．９％、８２．４％、５２．９％及２９．４％；只感染ＨＢＶ的分别为１１．１％、２２．２％、１１．１％和１１．１％；仅摄入ＡＦＢ１的分别为１５．８％、２６．３％及１５．８％（ｐ２１）；空白对照无肝癌及上述基因蛋白表达。带瘤肝ＩＧＦ－Ⅱ、ＨＢｘＡｇ及ｐ２１检出率均显著高于无瘤者，而表达这３种基因蛋白的动物肝癌发生率也均明显高于阴性者。突变的ｐ５３蛋白表达仅见于７例（５８．３％）中低分化的肝癌。结论：ＨＢＶ与ＡＦＢ１共同激发这些基因的异常表达可能是其协同致肝癌机制之一。Purpose To study the mechanism of synergistic effect in hepatocarcinogenesis induced by HBV infection and aflatoxin B 1 intake. Methods Immunohistochemical staining was used in formalin fixed, paraffin embedded sections of cancer and liver tissues. The significance of the data was statistical evaluate by χ 2 test. Results The incidence of hepatocellular carcinoma(HCC) was 52 9% in experimental tree shrews received both HBV and aflatoxin B1 (AFB 1). It was significantly higher than that of animals exposed to HBV (11 1%,Group B), or aflatoxin B1(15 8%, Group C) alone. HCC was not found in control animals (Group D). The expressions of insulin like growth factor Ⅱ(IGF Ⅱ) were 82 4%,22 2%,26 3% and 0 in Group A, B, C and D respectively. The significant differences of IGF Ⅱ were observed between group A and B, C and D ( P <0 05). The expressions of p21 were 29 4%, 11 1%,15 8% and 0 in group A, B, C and D respectively. The positivity rate of HBxAg was significantly higher in Group A than that in Group B (52 9% vs 11 1%, P <0 05). The parallel relations between the incidence of HCC and the over expressions of these genes proteins have been found in each group. On the other hand, the expressions of these genes in tumor bearing tree shrews were significantly higher than that in no tumor bearing animals. The expression of p53 mutation was 58 3% in HCC of tree shrews. Conclusion These foundings suggest a synergistic effect of HBV and AFB 1 in activation of these genes in tree shrews. Over expressions of these genes may play an important role in the courses of hepatocarcinogenesis in tree shrews.

关 键 词：肝癌 肿瘤发生 HBXAG IGF-Ⅲ P21基因 P53基因 

分 类 号：R735.702[医药卫生—肿瘤] R73－354[医药卫生—临床医学]