低氧致鼠腺泡内肺动脉PDGF-B链蛋白表达的变化  

Hypoxia induced the expression of platelet derived growth factor (PDGF) B chain protein by intra acinar pulmonary arteries

作　　者：李丰[1,2] 车东媛[1,2] 邓仲端 

机构地区：[1]同济医科大学病理学教研室 [2]日本Mochida制药有限公司

出　　处：《临床与实验病理学杂志》1998年第4期382-384,I052,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：探讨血小板源性生长因子（ＰＤＧＦ）在低氧肺动脉高压血管构形重建发生中的作用。方法：应用免疫组化技术结合计算机图像分析，检测了低氧大鼠腺泡内肺动脉（ＩＡＰＡ）ＰＤＧＦ－Ｂ链蛋白表达水平。结果：常氧时，ＩＡＰＡ仅有ＰＤＧＦ－Ｂ链蛋白弱表达；低氧１天时，ＩＡＰＡ便有较强的ＰＤＧＦ－Ｂ链蛋白表达，定位于ＩＡＰＡ的内皮和中膜，低氧３天至１４天仅分布于中膜；低氧１、３、５、７、１４天各时间点ＰＤＧＦ－Ｂ链蛋白表达分别为常氧组的１．５３、１．５９、１．５６、１．６２和１．４２倍，差异有显著性（Ｐ＜０．０１）。Purpose To study the role for PDGF in mechanisms for vascular remodeling of hypoxic pulmonary hypertension. Methods Immunohistochemical technique combined with computer image analysis was used to determine expression of PDGF B chain protein by intra acinar pulmonary arteries (IAPA) in rat exposed to hypoxia. Results A weak PDGF B chain protein expression was observed in IAPA of rat in normoxia. However, a stronger PDGF B chain protein expression was found in endothelium and medium of IAPA in hypoxia for 1 day and only in medium of IAPA from day 3 to 14. Computer image analysis indicated that compared with normoxic group, the hypoxic group showed the 1 5,1 56,1 62, and 1 42 fold increase in the PDGF B chain protein content of IAPA, for 1,3,5,7 and 14 days, respectively ( P <0.01). Conclusion The increased PDGF B chain protein expression may participate in the process of vascular remodeling in pulmonary hypertension caused by hypoxia.

关 键 词：IAPA 肺动脉高压症 缺氧 PDGF-B 链蛋白 

分 类 号：R543.202[医药卫生—心血管疾病] R594.3[医药卫生—内科学]