两相体系中固定化红酵母细胞催化3,5-双三氟甲基苯乙酮的不对称还原反应  

Asymmetric Reduction of 3,5-Bistrifluoromethylphenyl Acetophenone by Immobilized Saccharomyces Rhodotorula in Biphasic System

作　　者：张芳[1,2] 薛颖[1] 李莉[1] 王旻[1] 

机构地区：[1]中国药科大学生命科学学院,江苏南京210009 [2]南京中医药大学药学院,江苏南京210046

出　　处：《药物生物技术》2010年第2期125-129,共5页Pharmaceutical Biotechnology

基　　金：2004年江苏省"六大人才高峰"资助项目

摘　　要：采用海藻酸钠固定红酵母细胞为生物催化剂,对有机溶剂—水两相体系不对称还原3,5-双三氟甲基苯乙酮合成(S)-3,5-双三氟甲基苯乙醇的影响因素进行了考察,并与静息细胞转化法进行比较。结果表明,固定化红酵母细胞在5%辛烷中进行转化,于30℃p、H 8、1.42 mg/mL底物浓度并添加葡萄糖作为辅助底物的条件下,转化效果最佳,经6次重复使用后,仍可保留50%的转化活力。The asymmetric reduction of 3, 5 - Bistrifluoromethyl acetophenone to （S）- 3, 5 - Bistrifluoromethyl acetophenol ethanol was catalyzed by immobilized Saccharomyces rhodotorula in organic solvent-aqueous hiphasic system. All the factors involved in the biocatalysis were investigated to investigate the optimum reaction conditions. The asymmetric reduction of 3, 5 - Bistrifluoromethyl acetophenone was influenced by several factors and the best biocatalystic results were obtained under the optimum conditions as follows： the organic solvents octane, the ratio of octane 5% at pH 7.0, 30 ℃ with the cell concentration 125 mg/mL and the substrate concentration 1.42 mg/mL while glucose as the co-sub- strate. The immobilized cells showed the stable catalytic activity with the 50% reserved activity and were superior especially in reusability in comparison with rest cells.

关 键 词：固定化 红酵母 3  5-双三氟甲基苯乙酮 (s)-3  5-双三氟甲基苯乙醇 不对称还原 

分 类 号：Q7[生物学—分子生物学]