响应面法优化蚕沙中叶绿素的微波提取工艺  

Optimization of Chlorophyll Microwave Extraction in Silkworm Faeces by Response Surface Methodology

作　　者：海洪[1] 金文英[1] 汪坤[1] 王新雯[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《桂林工学院学报》2010年第1期128-132,共5页Journal of Guilin University of Technology

基　　金：广西自然科学基金资助项目(桂科自0679001);广西教育厅科研项目(200911LX120)

摘　　要：为了节能、高效地从蚕沙中提取叶绿素,采用2∶1丙酮乙醇溶液为提取剂辅以微波处理,利用响应面法(RSM)优化工艺条件。在单因素试验的基础上,通过Box-Behnken中心组合试验设计(BBD)进行响应面分析。结果表明,微波提取蚕沙中叶绿素的最佳工艺条件为:微波辐射59 s,提取溶剂与蚕沙用量比例(液料比)27 mL/g,提取时间26 min,提取温度70℃,叶绿素提取率预测值为4.41 mg/g,验证值为4.26 mg/g,与预测值的相对误差为3.4%,与传统有机溶剂法相比,提取率提高了4.8倍。In the extraction of chlorophyll from silkworm faeces,the response surface methodology（RSM） is used to optimize the microwave extraction chlorophyll from silkworm faeces.The optimal chlorophyll extraction condition is determined by single factor experiment and response surface methodology（RSM） with Box-Behnken design（BBD）.The result shows that the optimal conditions for microwave extraction are：microwave radiation time 59 s,solvent-solid ratio 27 mL/g,extraction time 26 min,and extraction temperature 70 ℃.The predicted chlorophyll extraction yield is 4.41 mg/g,and the verification extraction yield is 4.26 mg/g,with relative error of 3.4%.It is improved by 4.8 times compared with traditional organic solvent extraction.

关 键 词：响应面法 蚕沙 叶绿素 微波提取 

分 类 号：TS202.3[轻工技术与工程—食品科学] S886.9[轻工技术与工程—食品科学与工程]