RUNX3、p21^(CIP1/WAF1)、cyclinD1在肝细胞性肝癌中的表达及其临床意义  

Expression and Clinical Significance of RUNX3,p21^(CIP1/WAF1),CyclinD1 Protein in Hepatocellular Carcinoma

作　　者：高锋利[1] 王淑静[2] 李昭宇[3] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学中心实验室,银川750004 [3]宁夏医科大学附属医院,银川750004

出　　处：《宁夏医科大学学报》2010年第2期165-168,共4页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金资助项目(NZ08109)

摘　　要：目的探讨RUNX3、p21CIP1/WAF1、cyclinD1蛋白在肝细胞性肝癌(HCC)和癌旁组织中的表达,以及三者在HCC组织中表达的相关性,三者的表达与临床病理因素之间的关系。方法采用回顾性分析方法,收集43例HCC新鲜标本及其癌旁组织,用免疫印迹(Western Blot)技术检测癌及癌旁组织中RUNX3、p21CIP1/WAF1、cyclinD1蛋白的表达。结果RUNX3、p21CIP1/WAF1蛋白在HCC组织中的相对表达值低于癌旁组织,两组比较差异有统计学意义(P<0.05);cyclinD1蛋白在肝癌组织中的相对表达值高于癌旁组织,差异有统计学意义(P<0.001);RUNX3、p21CIP1/WAF1c、yclinD1蛋白相对表达值均与TNM分期及组织分化程度有关(均P<0.05);HCC组织中RUNX3蛋白与p21CIP1/WAF1蛋白表达呈正相关(r=0.696,P<0.05),RUNX3蛋白与cyclinD1蛋白表达呈负相关性(r=-0.435,P<0.05)。结论RUNX3蛋白可能通过影响p21CIP1/WAF1和cyclinD1蛋白的表达影响肝细胞性肝癌的发生发展;联合检测RUNX3、p21CIP1/WAF1和cyclinD1蛋白的表达可能对HCC的诊断及预后判断有一定参考价值。Objective To investigate the expression and clinical significance of RUNX3,p21CIP1/WAF1,cyclinD1 protein in human hepatocellular carcinoma（HCC）.Methods Western Blot was used to detect RUNX3,p21CIP1/WAF1,cyclinD1 protein expression level in samples of hepatocellular carcinoma and corresponding para-cancer tissue,which were obtained from surgically resection specimens of 43 patients.Results Western Blot showed that the expression of RUNX3 and p21CIP1/WAF1 in hepatocellular carcinoma was significantly lower than those in corresponding paracancer tissue（P0.05） and the expression of cyclinD1 in hepatocellular carcinoma was significantly higher than that in corresponding para-cancer tissue（P0.05）.The depression of RUNX3 was correlated with TNM stage and pathologic differentiation degree（P0.05）.CyclinD1 and p21CIP1/WAF1expression was significantly associated with TNM stage and pathologic differentiation degree（P0.05）.The correlationship between RUNX3 and p21CIP1/WAF1 expression was significant（r=0.696,P0.5） and there was a significant inverse correlationship between RUNX3 and cyclinD1 expression（r=-0.435,P0.05）.Conclusion The abnormal expression of RUNX3,p21CIP1/WAF1,cyclinD1 gene in human hepatocellular carcinoma suggest that RUNX3,p21CIP1/WAF1,cyclinD1 gene may play an important roles in the development and progression of hepatocellular carcinoma.The combination detection of the three indices may provide reference to the diagnosis and evaluating the clinicopathological parameters of the hepatocellular carcinoma.

关 键 词：肝细胞性肝癌 RUNX3 P21CIP1/WAF1 CYCLIND1 

分 类 号：R735.7[医药卫生—肿瘤]