胃癌细胞HGC-27与SGC-7901体外增殖和黏附能力的比较  

Comparison of Proliferation and Adherence between Two Human Gastric Cancer Cell Lines in Vitro

作　　者：王婷婷[1] 王玉巧[1] 马佰菁[1] 韩梅[1] 林佳静[1] 孙伯坚[1] 

机构地区：[1]宁夏医科大学基础医学院病原生物学与免疫学系,银川750004

出　　处：《宁夏医科大学学报》2010年第2期169-171,共3页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金(NZ0772);宁夏高等学校科学研究项目(2009-47)

摘　　要：目的比较两株胃癌细胞HGC-27与SGC-7901的体外增殖和黏附能力。方法分别培养胃癌细胞HGC-27、SGC-7901,用噻唑蓝(MTT)比色实验和流式细胞术(FCM)比较两种细胞的体外增殖能力;用同质黏附和异质黏附实验比较两种细胞的体外黏附能力。结果MTT比色实验绘制生长曲线显示SGC-7901生长较HCC-27慢;流式细胞术结果表明SGC-7901的增殖活性低于HGC-27(P<0.05);黏附实验中SGC-7901同质黏附强于HGC-27,异质黏附弱于HGC-27(P<0.05)。结论胃癌细胞SGC-7901的体外增殖能力和异质黏附能力低于HGC-27,同质黏附能力高于HGC-27,提示胃癌细胞HGC-27的转移能力可能强于SGC-7901。Objective To compare the proliferation and adhesion between two gastric cancer cell lines in vitro.Methods Human gastric cancer cell lines HGC-27 and SGC-7901 were cultured.The abilities of proliferation were measured with MTT colorimetric assay and flow cytometry（FCM）;The ability of adhesion was detected using homogeneous array and heterogeneous array.Results The result of MTT colorimetric assay showed that the cellular growth of SGC-7901 was significantly slower than that of HGC-27（P0.05）.The SPF of HGC-27 showed significantly higher than that of SGC-7901（P0.05）.Homogeneity adhesion experiment suggested homogeneity adhesion of HGC-27 was significantly weaker than that of SGC-7901.Alloplasm adhesion experiments indicated that the adhesive activity of HGC-27 was significantly stronger than that of SGC-7901.Conclusion For SGC-7901,the ability of proliferation and homogeneous adhesion were lower than that for HGC-27,and the ability of heterogeneous adhesion in SGC-7901 was stronger than that in HGC-27.

关 键 词：胃癌细胞 HGC-27 SGC-7901 增殖 黏附 

分 类 号：R735.2[医药卫生—肿瘤]