松茸组织分离物的rDNA-ITS序列鉴定  

Identification of cultures isolated from fruit body of Tricholoma matsutake by rDNA-ITS sequence analysis

作　　者：张灵[1,2] 赵琪[1] 陈严平[1] 孟珍贵[1] 李荣春[1] 

机构地区：[1]云南农业大学食用菌研究所,昆明650201 [2]云南农业大学基础与信息工程学院,昆明650201

出　　处：《广西植物》2010年第2期181-184,共4页Guihaia

基　　金：云南省科技计划国际科技合作专项(2006GH06)~~

摘　　要：以采自云南丽江的松茸子实体为材料,进行组织分离后,利用一对ITS引物(ITS1-ITS4)对子实体(SR176B,SR172B)和分离物(SR176H,SR172H)进行了PCR扩增、琼脂糖凝胶电泳分析,得到了700bp左右的扩增条带,进一步对ITS序列进行同源性检索比对,结果表明SR176H与SR176B,SR172H与SR172B序列同源性均为100%,鉴定出该分离物就是松茸的纯培养物。A pair of general primer（ITS1- ITS4）was used to test in the amplification of the internal transcribed spacer（ITS）region of the chromosomal DNA of fruit body of Tricholoma matsutake of Lijiang and its isolates. The results analyzed by Agarose gel electrophoresis showed that the primers of ITS1 and ITS4 amplified about 700hp fragments for all samples. The ITS fragments of T. matsutake fruit body and its corresponding isolates were sequenced and aligned. Their sequences of SR176H and SR176B showed a 100% homogeneity,the same result also appeared between SR172 H and SR172B. Based on these facts, SR176 H and SR172 H were confirmed to be pure cultures of T. matsutake.

关 键 词：松茸 子实体 分离物 ITS序列 

分 类 号：Q949.32[生物学—植物学]