人工培养冬虫夏草胞外多糖的分离纯化研究  

Isolation and Purification of Exopolysaccharides from Cultured Cordyceps sinensis (Cs-HK1)

作　　者：闫景坤[1,2] 李琳[1] 吴建勇[2,3] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]香港理工大学深圳研究院分子药理重点实验室,广东深圳518064 [3]香港理工大学应用生物与化学科技学系

出　　处：《现代食品科技》2010年第4期366-369,共4页Modern Food Science and Technology

摘　　要：本文以乙醇沉淀法提取得到的人工培养冬虫夏草(Cs-HK1)粗胞外多糖为研究对象,采用DEAE-52纤维素(Cl-)离子交换柱层析和Sephadex G-100葡聚糖凝胶柱层析进行分离、纯化得到一水溶性胞外多糖分EPS-1A。紫外光谱、比旋光度和凝胶渗透色谱法研究表明该多糖为相对均一组分,中性糖含量为99.0%,重均相对分子质量为4.0×104。多糖特征反应表明该多糖为非淀粉类,不含单糖、糖醛酸、蛋白质、核酸和多酚类物质的中性多糖。In this paper, the crude exopolysaccharides was extracted from cultured Cordyceps sinensis （Cs-HK1） by ethanol precipitation. The water-soluble exopolysaccharide EPS-1A was obtained by isolation and purification using DEAE-52-Cellulose （Cl-） ion-exchange column chromatography and Sephadex G-100 gel column chromatography and identified with UV spectrum, optical rotation and gel permeation chromatography （GPC）. Results suggested that EPS-1A was a relatively homogeneous component, containing 99.0% neutral sugar and its weight average molecular weight was of 4.0×10^4. Characteristic reactions of polysaccharides identified that EPS-1A was a non-starch neutral exopolysaccharides, which did not contain monosaccharides, uronic acids, protein, nucleic acids and polyphenols.

关 键 词：冬虫夏草(Cs-HK1) 胞外多糖 柱层析 DEAE-52纤维素 SEPHADEX G-100 

分 类 号：S567.35[农业科学—中草药栽培]