姜黄素对bFGF诱导的人乳腺癌细胞MDA-MB-231增殖的影响  

Effects of curcumin on proliferation of human breast carcinoma cells induced by bFGF

作　　者：林苗苗[1] 王静[1] 林绍强[1] 李校堃[1] 蔡琳[1] 

机构地区：[1]温州医学院药学院,325035

出　　处：《浙江医学》2010年第2期223-225,共3页Zhejiang Medical Journal

摘　　要：目的 通过观察姜黄素（Cur）对碱性成纤维细胞生长因子（bFGF）诱导人乳腺癌细胞（MDA-MB-231）增殖的影响,探讨Cur治疗乳腺癌的作用机制.方法 将人乳腺癌细胞MDA-MB-231用含10%胎生血清的RPMI-1640培养基培养.同时根据加入Cur的不同浓度将实验分为7组：空白对照组、bFGF组（bFGF 5 ng/ml）、干预1组（bFGF 5ng/ml＋Cur 1 μmol/L）、干预2组（bFGF 5ng/mI＋Cur 2.5 μmol/L）、干预3组（bFGF 5 ng/ml＋Cur 5 μmol/L）、干预4组（bFGF 5ng/ml＋Cur 10 μmol/L）、干预5组（bFGF 5 ng/ml＋Cur 20 μmol/L）,并在倒置显微镜下观察各组细胞形态,通过MTT法检测Cur对细胞增殖的影响.结果 bFGF组可显著促进人乳腺癌细胞（MDA-MB-231）的增殖,与对照组的差异有统计学意义（P〈0.05）,且其作用随着处理时间的延长而增强.各干预组均可显著抑制人乳腺癌细胞（MDA-MB-231）的增殖,且呈时间和浓度依赖性.结论 Cur可有效抑制bFGF诱导的人乳腺癌细胞增殖,可能为其用于乳腺癌治疗的机制所在.Objective To investigate the effects of curcumin on proliferation of human breast carcinoma cells induced by bFGF. Methods Cultured breast carcinoma MDA-MB-231 cells were divided into 7 groups： the blank control group, bFGF group （5 ng/ml of bFGF）, intervention group 1 （5 ng/ml of bFGF ＋ 1 p tooL/L of curcumin）, intervention group 2 （5 ng/ml of bFGF ＋ 2.5 p tooL/L of curcumin）, intervention group 3 （5 ng/ml of bFGF ＋ 5 p tooL/L of curcumin）, intervention group 4 （5 ng/ml of bFGF ＋ 10 p tooL/L of curcumin） and intervention group 5 （5 ng/ml of bFGF ＋ 20 p tooL/L of curcumin）. The morphological changes of cells in each group were observed with inverted microscope and the cell proliferation was determined by MTT assay. Results The proliferation of MDA-MB-231 cells was induced significantly by 5 ng/mL of bFGF compared with the blank control group （P〈0.05） and this effect was enhanced with time extension. However, the proliferation effect on MDA-MB-231 cells was inhibited significantly by curcumin in a time-and concentration-dependent manner. Conclusion Curcumin inhibits the proliferation of human breast carcinoma cells induced by bFGF, which is associated with the cell apoptosis.

关 键 词：姜黄素 BFGF 人乳腺癌细胞 增殖 

分 类 号：R737.9[医药卫生—肿瘤]