细胞外信号调节激酶通路抑制剂对完全弗氏佐剂致痛大鼠背根神经结中谷氨酸转运蛋白表达的影响  

Effect of ERK pathway inhibitor on the expression of glutamate transporter in dorsal root ganglion of rats with complete Freund's adjuvant-induced allodynia

作　　者：苏小军[1] 郝建华[1] 郭正刚[1] 李萌萌[1] 

机构地区：[1]解放军总医院第一附属医院麻醉科,北京市100037

出　　处：《实用医学杂志》2010年第7期1128-1130,共3页The Journal of Practical Medicine

摘　　要：目的:观察细胞外信号调节激酶(ERK)通路抑制剂PD98059对完全弗氏佐剂(CFA)致痛大鼠脊髓背根神经结(DRG)中谷氨酸转运蛋白表达的影响。方法:24只大鼠随机分为对照组、单纯致痛组、PD1组和PD10组。疼痛模型采用大鼠足底注射CFA100μL。对照组及单纯致痛组经椎管内给予二甲基亚砜(DMSO)10μL,每日2次。PD1组和PD10组分别经椎管内给予PD980591μg或10μg,每日2次。测定大鼠每日痛阈变化,3d后RT-PCR法检测大鼠DRG内谷氨酸转运蛋白表达的变化。结果:注射CFA致痛后各组大鼠痛阈均显著降低,PD1组与PD10组大鼠痛阈均较单纯致痛组显著升高,PD1组与PD10组痛阈升高差异无显著性。单纯致痛组DRG中谷氨酸转运体1(GLT-1)和兴奋性氨基酸载体1(EAAC1)表达显著高于对照组、PD1组、PD10组。除PD1组DRG中GLT-1表达高于对照组外,PD1组、PD10组及对照组DRG中GLT-1与EAAC1表达差异无显著性。结论:鞘内给予PD98059可减轻大鼠足底注射CFA引起的疼痛反应并减低致痛侧DRG中谷氨酸转运蛋白表达。Objective To observe the effect of ERK pathway inhibitor PD98059 on the expression of glutamate transporter in dorsal root ganglion of rats with complete Freund＇s adjuvant （CFA）-induced allodynia. Methods 24 rats were divided into 4 groups： control group, pure pain group, PD1 group, and PD10 group, each n = 6. 18 rats in the later 3 groups were assigned to receive injection of 100 uL of CFA at foot sole while 6 rats in control group received injection of 100 uL of normal saline. Then rats in control group and pure pain group received 10 uL of DMSO in spinal canal twice a day, and those in PD1 group and PD10 group received 1 ug or 10 ug of PD98059 in spinal canal twice a day. Pain threshold was tested everyday, and the expression of glutamate transporter-1 （GLT-1） in dorsal root ganglion was measured in the 4 groups 3 days later. Results The pain threshold was decreased significantly in the 3 groups except control group, and was significantly lower in pure pain group as compared to PD1 group and PD10 group （P 〈 0.05）, but no significant difference was observed between the later 2 groups. The expressions of GLT-1 and excitatory amino acids carrier 1 （EAAC1） in dorsal root ganglion in pure pain group were significant higher than those in the other 3 groups. The expression of GLT-1 in dorsal root ganglion in PD1 group was significantly higher than that in control group. Conclusion PD98059 given intrathecally can reduce the CFA-induced allodynia and decrease the expression of GLT-1 in dorsal root ganglion of rats.

关 键 词：钙-钙调素依赖性蛋白激酶类 谷氨酸 载体蛋白质类 炎性疼痛 完全弗氏佐剂 

分 类 号：R741[医药卫生—神经病学与精神病学]