淡水湖泊中类蛭弧菌的分离方法  

Study of a Method to Isolate the Bdellovibrio-and-Like Organisms(BLAOs) from the Freash Water Lakes

作　　者：曾佳[1,2] 梁小兵[1] 陆婷[1,2] 

机构地区：[1]中园科学院地球化学研究所环境地球化学国家重点实验室,贵州贵阳550002 [2]中国科学院研究生院,北京100039

出　　处：《矿物学报》2010年第1期77-82,共6页Acta Mineralogica Sinica

基　　金：贵州省科学技术基金(20092040);国家自然科学基金项目(40473050);国家重大基础研究项目(2006CB4003200)

摘　　要：从贵州阿哈湖、百花湖及云南滇池不同深度的湖水中筛选出2株宿主菌株。经16SrRNA基因序列分析,这2株宿主菌分别鉴定为肠杆菌(Enterobacteriales)和黄色单胞菌(Xanthomonadales)。分别利用这2株宿主菌,从湖水样品中获得到了类蛭弧菌噬菌斑。通过吸光度分析、用淡水类蛭弧菌的特异性引物进行基因扩增、DNA序列分析等一系列方法,证实了所分离的菌株为类蛭弧菌。建立了用同一生长环境中的宿主菌来分离类蛭弧菌的方法,并用该方法从淡水湖泊中分离出了不同类群的类蛭弧菌菌株。Two kinds of preys were isolated from different water depths of Lake Aha and Baihua in Guizhou Province and Lake Dianchi in Yunnan Province.They are identified as Enterobacteriales and Xanthomonadales respectively by 16S rDNA sequence analysis.The plaques of BALOs were got from lake water samples.The isolates were verified as BALOs though absorbency determination,PCR with specific primers and DNA sequence analysis.A method of isolating BALOs by using isolated preys in the same inhabitants was established.Various clusters of BALOs were isolated from freshwater lakes by using the methods.

关 键 词：类蛭弧菌 分离 宿主菌 PCR DNA序列分析 

分 类 号：Q939.99[生物学—微生物学] X172[环境科学与工程—环境科学]