负载肝癌抗原树突状细胞瘤苗抑制肝癌细胞免疫作用研究  

ANTITUMOR IMMUNITY EFFECTS OF DENDRITIC CELL VACCINE LOADED WITH HEPATOCELLULAR CARCINOMA ANTIGEN

作　　者：马玉强[1] 李志梁[1] 张明辉[1] 胡万宁[2] 

机构地区：[1]华北煤炭医学院附属肿瘤医院感染性疾病科,河北唐山063000 [2]华北煤炭医学院附属肿瘤医院头颈外科,河北唐山063000

出　　处：《河北医科大学学报》2010年第4期384-387,共4页Journal of Hebei Medical University

基　　金：河北省唐山市科学技术研究与发展指导计划项目(08130209d)

摘　　要：目的探讨人自体肝癌细胞裂解物致敏的树突状细胞(dendritic cell,DC)瘤苗对自体肝癌细胞免疫功能的影响。方法从肝癌患者外周血单个核细胞中诱导DC,用相关细胞因子及自体肝癌细胞刺激活化,制备DC瘤苗:应用流式细胞仪检测T淋巴细胞增殖情况,观察刺激术前及术后自体的T淋巴细胞以及产生的特异性细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)对自身肿瘤细胞的抑制效果。结果肝癌DC瘤苗刺激术后自体T淋巴细胞增殖能力及抑制肿瘤细胞作用显著强于术前,T淋巴细胞增殖指数术后1.88±0.15较术前1.18±0.13显著增高、CD8^+T细胞增殖指数术后1.55±0.35较术前0.95±0.12显著增加,术后与术前比较差异有统计学意义(P<0.05)。肝癌DC瘤苗活化的术后自体CTL对自体肝癌细胞有较强杀伤作用,杀伤率由术前的(30.35±3.1 6)%增加到术后的(72.80±3.55)%,差异有统计学意义(P<0.01)。结论肝癌DC瘤苗活化的术后自体CTL对自体肝癌细胞杀伤作用比术前作用显著,DC瘤苗能有效地诱导抗肿瘤免疫反应,有望在肝癌术后的治疗中发挥作用。Objective To investigate the effects of dendritic cell （DC） pulsed with autologous hepatoma cell lysates on the immune function of the postoperatively patients with hepatocellular carcinoma（HCC）. Methods DC isolated from peripheral blood mononuclear cell of HCC patients were cultured and then were pulsed with autologous hepatoma cell lysates and related factors. The proliferation of T lymphocytes was detected by flow cytometry. The inhibitory effect of T-lymphocytes and special cytotoxic T lymphocyte （CTL） on HCC were observed postoperatively and preoperatively. Results The proliferation of T lymphocyte stimulated by autologous DC vaccine and the inhibition to tumor cells were stronger than those of preoperation. Postoperative T lymphocyte proliferation index 1.88±0.15 was significantly higher than that of preoperation 1.18 ±0.13. Postoperative CD8^＋ cell proliferation index 1.55 ±0.35 increased significantly compared with that of preoperation 0.95 ± 0.12 and the differences were statistically significant（ P 〈0.05）. Postoperative autologous CTL which was activated by HCC DC vaccine had strong killing effect against autologous HCC cells, the kill rate increased from preoperation （30.35± 3.16）% to postoperative （72.80±3.55）%. Conclusion The killing effect of postoperative autologous CTL against autologous HCC cells was stronger than that of preoperation,DC vaccine can effectively induce anti-tumor immune response in liver cancer is expected to play a role in the postoperative treatment.

关 键 词：肝肿瘤 树突细胞 免疫活性 

分 类 号：R735.7[医药卫生—肿瘤]