人硫酸酯酶1对生长因子介导的胰腺癌细胞生长的作用  

Effect of human sulfatase 1 on the growth factor-mediated cell growth in pancreatic cancer cells

作　　者：郝建军[1] 多永胜[1] 杨钰[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院普外科,黑龙江齐齐哈尔161000

出　　处：《现代医学》2010年第2期125-128,共4页Modern Medical Journal

摘　　要：目的:探讨人硫酸酯酶1对生长因子诱导下胰腺癌细胞生长作用的影响及其机制。方法:应用lipo-fectam ine方法转染人硫酸酯酶1基因到低表达该基因的胰腺癌细胞Panc-1,通过Northern bloting筛选阳性转染的细胞克隆,并检测转染后胰腺癌细胞硫酸酯酶1的水平;应用噻唑氮蓝比色分析(MTT)法检测转染后胰腺癌细胞对生长因子作用的影响;应用W estern bloting检测人硫酸酯酶1转染对生长因子受体与MAPK磷酸化水平的影响。结果:硫酸酯酶1稳定转染入Panc-1细胞后,Panc-1细胞稳定表达硫酸酯酶1 mRNA,并且硫酸酯酶1表达水平升高。FGF-2、HB-EGF、EGF均促进胰腺癌细胞Panc-1的生长,硫酸酯酶1基因转染后减弱FGF-2对细胞的生长促进作用,降低FGF-2所诱导的MAPK磷酸化水平,但对HB-EGF、EGF的作用无明显影响。结论:人硫酸酯酶1转染减弱生长因子FGF-2所诱导的胰腺癌细胞的生长促进作用;人硫酸酯酶1可能参与胰腺癌的病理发生发展过程。Objective： To investigate the effect of newly identified human sulfatase 1 gene on the growth factorinduced cell growth in pancreatic cancer cells. Methods： Human sulfatase 1 gene was stably transfected into Panc-1 cells. Northern blot was carried out to confirm the transfection and positive transfected colony was detected and selected. Human sulfatase activity was measured. Cell growth ability was measured by MTT assay and growth factor receptor phosphorylation and MAPK phosphorylation were determined by Western blot. Results： After human sulfatase-1 transfeetion, Panc-1 cells showed stable human sulfatase-1 mRNA expression and increased sulfatase activity, which reduced the FGF- 2- induced cell proliferation ability and FGF- 2- induced MAPK phosphorylation, but there was no prominent effect on HB-EGF and EGF function and receptor phosphorylation. Conclusion： Human sulfatase 1 expression can decrease FGF-2-induced cell growth and attenuate FGF-2-induced MPAK phosphorylation, which may play a role in pancreatic cancer pathogenesis.

关 键 词：胰腺癌 人硫酸酯酶1 生长因子 

分 类 号：R735.9[医药卫生—肿瘤]