应用AFLP建立玉米基因组DNA指纹图谱的研究  

Research on AFLP Analysis Systems Using Single and Double Restricted Enzyme in Maize

作　　者：马建岗[1] 周立全[1] 何会[1] 王辉[1] 

机构地区：[1]西安交通大学生命科学与技术学院,西安710049

出　　处：《生命科学仪器》2010年第2期49-51,共3页Life Science Instruments

基　　金：陕西省科学技术研究发展计划项目(2007K02-07);陕西省自然科学基金(2006C132)资助

摘　　要：分别采用PstI单酶切AFLP和EcoRI/MseI双酶切AFLP建立6个玉米品系的AFLPDNA指纹图谱,并进行亲缘关系鉴定。相比之下,PstI单酶切AFLP法较简单、经济,而EcoRI/MseI双酶切AFLP法扩增出的条带数量多、背景低;二者均适用于亲缘关系鉴定。AFLP analysis systems using PstIsingle and EcoRI/MseIdouble restricted enzymes were established and applied in 6 different cnl- tivars of maize to identify their genetic relationships. The results of the two methods were compared. Pstl AFLP is simple and economical, and EcoRI/MseIAFLP is sharp and informative. This suggests that they are both effective for assessing genetic relationships.

关 键 词：玉米 单酶切 双酶切 AFLP 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]