镧(Ⅲ)离子对菲律宾蛤仔不同组织金属硫蛋白诱导的研究  

Induction of Lanthanum(Ⅲ) ion to Metallothionein Synthesis in Different Organisms of Ruditapes Philippinarum

作　　者：孟范平[1] 付海防[1] 赵顺顺[1] 肖静[1] 高鹰[1] 

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100

出　　处：《中国稀土学报》2010年第2期232-237,共6页Journal of the Chinese Society of Rare Earths

摘　　要：稀土离子能否诱导海洋生物的金属硫蛋白（MT）合成,将影响到MT对海水重金属的指示作用。以菲律宾蛤仔（Ruditapes philippinarum）为试验动物,采用含镧（Ⅲ）离子（La3＋）的人工海水对其进行暴露培养,测定得到蛤仔鳃部和内脏中MT含量随暴露浓度和时间的变化。结果表明,La3＋能够促进蛤仔MT的诱导,当以1×102μg.L-1La3＋对蛤仔暴露培养5 d时,鳃部和内脏MT含量均达到最高水平,分别为0.135±0.014μmol-SH/g和0.276±0.025μmol-SH/g;1×10-1~1×102μg.L-1的La3＋对鳃部MT的诱导作用较大,继续增加La3＋浓度,诱导作用有所降低;1×10-1~1×104μg.L-1的La3＋对内脏MT均有较好诱导,并基本保持恒定,表明La3＋对机体MT的诱导具有一定限度。值得注意的是,我国某些海域的海水稀土离子浓度已达到可诱导双壳类MT合成的水平。Whether lanthanide ions could induce metallothionein（MT） synthesis in marine organisms might limit the use of MT as a biomarker of heavy metal pollution in seawater.Using Ruditapes philippinarum as an experimental animal,the influences of exposure concentration and duration were studied in relation to MT content in the gills and viscera after lanthanum（Ⅲ） ion（La3＋）-exposed.The results indicated that La3＋ exposure could induce MT synthesis,and the MT content reached the highest level after exposed to La3＋（1×102 μg·L-1） for 5 d（gills,0.135±0.014 μmol-SH/g;viscera,0.276±0.025 μmol-SH/g）.For gills,the MT production was accelerated by La3＋ of 1×10-1~1×102 μg·L-1 obviously,but decreased when exposed to higher concentration of La3＋.For viscera,all La3＋ treatments from 1×10-1 μg·L-1 to 1×104 μg·L-1 had significant induction of MT which contents were almost constant.This suggested that there was certain limit in inducing MT synthesis when exposed to La3＋.It was noticeable that the concentration of rare earth elements（REEs） in some coastal water of China had reached the level to induce MT synthesis in bivalves.

关 键 词：镧(Ⅲ)离子(La3+) 金属硫蛋白(MT) 诱导 菲律宾蛤仔 稀土 

分 类 号：X171.5[环境科学与工程—环境科学]