重组鸡IL-18与新城疫HN基因融合蛋白在毕赤酵母中表达条件的优化  

Optimization of Condition for Expression of Recombinant ChMIL18-HN in Pichia Pastoris

作　　者：王学强[1] 程相朝[1] 刘一尘[1] 李正[1] 张春杰[1] 郁川[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《河南科技大学学报（自然科学版）》2010年第2期73-76,共4页Journal of Henan University of Science And Technology:Natural Science

基　　金：河南省自然科学基金项目(0511032300)

摘　　要：为探索ChMIL18-HN在毕赤酵母中最适宜的表达条件,采用小型摇瓶培养系统,通过对重组毕赤酵母菌X-33/pPICZαA-ChMIL18-HN在不同诱导时间、培养液pH值、甲醇诱导剂剂量及培养温度的条件下表达目的蛋白的分析,以对其表达条件进行优化。结果表明:重组菌X-33/pPICZαA-ChMIL18-HN在pH6.5,甲醇诱导浓度为1.5%,培养诱导温度为30℃的条件下诱导156h,目的蛋白的表达量最高。To research the most optimization condition for expression of recombinant ChMIL18-HN in Pichia Pastoris,the P.pastoris X-33/pPICZαA-ChMIL18-HN was expressed in a small flask culture system.By studying the yield of target protein under different time of methanol inducing expression,different pH of medium,different inducing dose of methanol and different inducing temperature,the most appropriate expression conditions were determined.The results indicated that recombinant fusion protein was expressed to be the highest under pH6.5,methanol concentration 1.5%,culture-inducing temperature 30 ℃ for 156 hours.

关 键 词：基因 毕赤酵母 诱导表达 条件优化 

分 类 号：S852.4[农业科学—基础兽医学]