骨形成蛋白与胶原基纳米骨修复牙周骨缺损的实验研究  

The experimental study of periodontal bone defects repair with bone morphogenetic protein-nano-HAP-collagen

作　　者：范月静[1] 王春兰[1] 王英[1] 陈晶晶[1] 蒲勤 

机构地区：[1]天津医科大学第二医院口腔科,300211 [2]北京森康生物技术开发有限公司

出　　处：《中华口腔医学研究杂志（电子版）》2010年第2期4-7,共4页Chinese Journal of Stomatological Research(Electronic Edition)

摘　　要：目的探讨重组人骨形成蛋白-2(rhBMP-2)和胶原基纳米骨复合材料(nHAC)修复牙周骨缺损的效果。方法制备小型猪牙周骨缺损模型,设立rhBMP-2-nHAC复合材料植入组、空白对照组和单纯植入胶原基纳米骨组,术后8周观察各组间修复效果及组织病理学变化,通过骨组织形态计量学测定分析评价牙周骨组织的再生情况。结果术后8周可见复合材料植入组大量新生牙周组织生长,四环素单标记表面、四环素双标记表面、骨矿化沉积率、骨体积、类骨质表面、成骨细胞表面等骨组织形态计量学结果与空白对照组和单纯胶原基纳米骨组比较,差别均有统计学意义(P<0.05)。结论rhBMP-2和nHAC可明显促进牙周骨缺损修复。Objective To observe and evaluate the effects of recombinant human bone morphogenetic protein-2 （rhBMP-2） and nano-HAP-Collagen （nHAC） on periodontal bone defects repair. Methods The rhBMP-2 combined with nHAC were transplanted into man-made periodontal bone defects, and the groups covered with only nHAC or with nothing were the controls. The animals were sacrificed after 8 weeks and the periodontal bone regeneration was observed histologically. Bone histomorphometry was measured. Results The rhBMP-2-nHAC group exhibited a large amount of new periodontal tissue. In the bone histomorphometry study, significant differences were found for the STS, DTS, MAR, TBV, TOS and TBOS values among the three groups （P 〈 0.05）. The values of rhBMP-2- nHAC and nHAC group were significantly higher than that of the control group （P 〈 0.05）. The difference of those value were statistically significant between rhBMP-2-nHAC and nHAC group, too （P 〈 0.05）. Conclusions The rhBMP-2-nHAC compound material presents better osteoeonduetive and osteoinductive activity. It can be used to promote periodontal regeneration.

关 键 词：牙周骨缺损 重组人骨形成蛋白-2 胶原基纳米骨 骨组织形态计量学 

分 类 号：R783[医药卫生—口腔医学]