成人急性白血病患者FLT3基因及FLT3／ITD突变  

FLT3 gene and FLT3/ITD mutation in adult acute leukemia

作　　者：张丽晶[1] 贾瑞萍[1] 赵雪芸[1] 

机构地区：[1]内蒙古包头市中心医院,包头014040

出　　处：《国际输血及血液学杂志》2010年第2期113-115,共3页International Journal of Blood Transfusion and Hematology

摘　　要：目的 研究FLT3基因及FLT3基因内部串联重复（internal tandem duplication，ITD）突变（FLT3／JTD突变）与蒙古族成人急性白血病的关系，探索其在急性白血病的诊断及基因分型中的意义。方法采用聚合酶链反应（PCR）技术检测56例蒙古族成人初发急性白血病、10例蒙古族成人非白血病患者FLT3基因表达及FLT3／ITD突变情况。结果41例初诊急性髓细胞白血病（AML）患者中FLT3基因表达为70．7％（29／41）；FLT3／ITD突变阳性率为19．5％（8／41）。15例初诊急性淋巴细胞白血病（ALL）患者中FLT3基因表达为60．0％（9／15），15例ALL患者均未检测到FLT3／ITD基因突变。10例对照均无FLT3基因表达。结论FLT3基因在蒙古族成人急性白血病患者中有较高表达，FLT3基因的检出可能有助于急性白血病的诊断。ALL患者一般无FLT3／ITD突变，推测FLT3／ITD突变检测有助于急性白血病基因分型。Objective To analyze the relationship between FLT3 gene and FLT3 gene internal tandem duplication mutation （FLT3/ITD） with Mongolian adult acute leukemia, and explore the significance of FLT3 gene and FLT3/ITD in diagnose and genotyping of acute leukemia. Methods PCR was used to detect the FLT3 gene and the FLT3/ITD in 56 Mongolian adult acute primary leukemia patients and 10 Mongolian adult acute non-leukemia patients. Results The expression of FLT3 gene was detected in 70.7o/00 （29/41） primary acute myeloid leukemia （AML） cases and 19. 5% （8/41） was found with FLT3/ITD mutation. FLT3 gene expression in 15 primary acute lymphoblastic leukemia （ALL） was also found a high level of 60.0% （9/15）. FLT3/ITD mutation was not found in ALL patients. There was no FLT3 gene expression in control grope. Conclusion For a high level of the FLT3 gene expression in Mongolian adult acute leukemia, FLT3 gene detection might be helpful for diagnosing acute leukemia. FLT3/ITD mutation might be helpful to identify the genic characterization of acute leukemia.

关 键 词：白血病 蒙古族 FLT3基因 FLT3/ITD 

分 类 号：R733.71[医药卫生—肿瘤]