脂多糖对人牙周膜细胞COX-2蛋白表达的影响  

Lipopolysaccharide-induced cyclooxygenase-2 protein expression in human periodontal ligament fibroblasts

作　　者：张晓蕾 梁海 苗琪 钱起丰[2] 

机构地区：[1]深圳市南山区蛇口人民医院口腔科,广东深圳518067 [2]深圳市慢性病防治院中心实验室,广东深圳518000

出　　处：《临床口腔医学杂志》2010年第3期138-141,共4页Journal of Clinical Stomatology

摘　　要：目的:观察脂多糖对人牙周膜成纤维细胞COX-2表达的影响,探讨牙周病发生发展过程中脂多糖(LPS)与COX-2的关系。方法:使用不同浓度LPS刺激体外培养人牙周膜成纤维细胞,免疫组织化学法检测细胞COX-2蛋白表达,灰度分析并进行统计学处理。结果:LPS组COX-2蛋白表达明显高于对照组,并且LPS浓度越高,COX-2表达越强。结论:LPS能够诱导人牙周膜成纤维细胞COX-2表达上调,随着LPS刺激浓度增高,COX-2表达逐渐增强,这一机制很可能在牙周炎发生发展过程中扮演重要角色。Objective：To study lipopolysaccharide-induced cyclooxygenase-2 protein expression by the stimulate of lipopolysaccharide in human periodontal ligament fibroblasts（HPDLCs）and analyse the relationship with periodontitis.Method：HPDLCs was cultured in vitro,stimulated by lipopolysaccharide of varial viscosity,Then we test the expression of COX-2 by using immunohistoche staining and determination.Result：The expression of COX-2 with the stimulas of LPS is higher than the group of contrast.The thicker LPS is taken,the higher expression of COX-2 is.Conclusion：LPS may induce the expression of COX-2 in HPDLCs and with the thicker LSP taken,the higher expression of COX-2 is,which plays a very important role in periodontitis.

关 键 词：人牙周膜成纤维细胞 脂多糖 环氧合酶-2 

分 类 号：R781.4[医药卫生—口腔医学] Q786[医药卫生—临床医学]