Quantum Dots as Fluorescent Labels for Detection of Heat Shock Protein in Tumor Tissue Using Laser Scanning Confocal Microscope  

作　　者：杨欢 张杰 

机构地区：[1]Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [2]Department of Urology, Renmin hospital of Wuhan University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2010年第2期257-259,共3页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China (No.303711325)

摘　　要：A new Quantum Dots（Qdots） nanocrystal composed of semiconductor core and zinc sulfide shell, and its feasibility as labels in immunofluorescence analysis for the imaging of tumor biomarkers by laser scanning confocal microscope（LSCM） was investigated. Qdots taged by mercaptoacetic acid were conjugated with second antibody, then imaging differences of Heat Shock Proteins 70（HSP70） in renal carcinoma tissure sections with immunofluorescence analysis method using Qdots bioconjugates and conventional organic dye FITC were observed by LSCM to assess the brightness and opticalstability of Qdots. The experimental results showed Qdots bioconjugates achieved the better results in demonstrating HSP70 with more brighter color and more clear picture than FITC labels. Moreover, the label signals of Qdots did not fade clearly after continued exposure to a 488 nm laser for 1 h. The Qdots bioconjugates have good feasibility in immunofluorescence analysis for the bioimaging by LSCM.A new Quantum Dots（Qdots） nanocrystal composed of semiconductor core and zinc sulfide shell, and its feasibility as labels in immunofluorescence analysis for the imaging of tumor biomarkers by laser scanning confocal microscope（LSCM） was investigated. Qdots taged by mercaptoacetic acid were conjugated with second antibody, then imaging differences of Heat Shock Proteins 70（HSP70） in renal carcinoma tissure sections with immunofluorescence analysis method using Qdots bioconjugates and conventional organic dye FITC were observed by LSCM to assess the brightness and opticalstability of Qdots. The experimental results showed Qdots bioconjugates achieved the better results in demonstrating HSP70 with more brighter color and more clear picture than FITC labels. Moreover, the label signals of Qdots did not fade clearly after continued exposure to a 488 nm laser for 1 h. The Qdots bioconjugates have good feasibility in immunofluorescence analysis for the bioimaging by LSCM.

关 键 词：quantum dots immunofluorescence analysis BIOIMAGING laser scanning confocal microscope 

分 类 号：R730.4[医药卫生—肿瘤]