siRNA抑制喉鳞状细胞癌Hep-2细胞生长的作用  

Inhibition of growth and proliferation of Hep-2 cells by targeting hTERT gene using small interference RNA technology

作　　者：贾海英[1] 张涛[1] 唐智[2] 王继群[3] 

机构地区：[1]广州暨南大学附属第一医院耳鼻咽喉头颈外科,广州510632 [2]顺德市第一人民医院耳鼻咽喉头颈外科 [3]广州暨南大学医学院

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第7期308-310,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广东省自然科学基金资助项目(No:9451063201003299)

摘　　要：目的:探讨靶向人端粒酶基因hTERT对人喉鳞状细胞癌Hep-2细胞生长增殖的抑制作用。方法:根据人端粒酶基因hTERT设计和合成人端粒酶siRNA和随机的siN.C作为阴性对照,通过转染载体Lipofectamine 2000脂质体转染Hep-2细胞,进行形态学、RT-PCR和MTT法检测,观察其干扰效果。结果:①siRNA能够成功转染进入Hep-2细胞;②RT-PCR结果显示转染组与阴性对照组和空白组相比,hTERT mRNA表达明显减弱,转染组hTERT mRNA较空白组相对下降70%;③形态学和MTT结果显示人端粒酶siRNA能有效抑制Hep-2细胞增殖,抑制率达到65%。结论:靶向hTERT的siRNA能显著抑制喉鳞状细胞癌Hep-2细胞的生长增殖。Objective：To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.Method：siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase（RT-PCR）,MTT assay,and morphology.Result：①siRNA were transfected into Hep-2 cells successfully;②RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA;③The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.Conclusion：siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.

关 键 词：喉肿瘤 SIRNA 基因治疗 

分 类 号：R739.65[医药卫生—肿瘤]