细胞浆内Connexin32蛋白增强肝癌细胞Li-7的运动和侵袭能力  

Connexin32 Accumulation in the Cytoplasm Facilitates Motility and Invasiveness of Li-7 Hepatocellular Carcinoma Cells

作　　者：李庆昌[1] 高辉[1] 谢成耀[1] 刘树立[1] 邱雪杉[1] 王恩华[1] 

机构地区：[1]中国医科大学基础医学院病理教研室及附属第一医院病理科,沈阳市110001

出　　处：《中国肿瘤临床》2010年第7期369-371,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30700806);教育部留学归国人员科研启动基金资助(编号:2008-890)~~

摘　　要：目的:探讨肝癌细胞中间隙连接蛋白Connexin32对肝癌细胞运动和侵袭能力的影响。方法:利用逆病毒感染的方法在肝癌细胞系Li-7中建立Connexin32蛋白表达可通过doxycycline调控的Li-7 Tet-off Cx32稳定克隆,运用体外transwell migrationandinvasion assay方法验证Connexin32表达与肝癌细胞运动和侵袭能力的相关性。结果:建立稳定整合了Connexin32 cDNA和调控序列Tet-off的肝癌细胞Li-7亚克隆,Western-blot结果显示外源性Connexin32蛋白表达于细胞浆中,亚细胞定位分析证实Connexin32蛋白定位于高尔基复合体内,此细胞浆内Connexin32蛋白过表达显著增强肝癌细胞的体外运动和侵袭能力。结论:肝癌细胞浆内异位表达的Connexin32蛋白发挥促进肿瘤进展的作用。Objective： To explorer the effects of gap junction protein connexin32 on motility and invasiveness of hepatocellular carcinoma cells. Methods： The stable subclone Li-7 Tet-off Cx32 in which connexin32 expression could be controlled by doxycyclin was established in hepatocellualr carcinoma Li-7 cells by retrovirus infection. Cell motility and invasive ability was analyzed by in vitro transwell motility and invasion assay. Results： A subclone originated from Li-7 cells was established, in which both connexin32 cDNA and regulatory element Tet-off were stably integrated. Western-blot results showed that exogeneous connexin32 was expressed in the cytoplasm, mainly＇in Golgi apparatus, and the abnormal accumulation of connexin32 protein in the cytoplasm could significantly promote motility and invasiveness of Li-7 cells. Conclusion： Cytoplasmic accumulation of connexin32 protein can promote tumor progression in Li-7 hepatocellular carcinoma cells.

关 键 词：间隙连接蛋白 肝癌 肿瘤进展 

分 类 号：R-5[医药卫生]