虎杖配伍山楂抗动脉粥样硬化易损斑块病变的机制研究  

Effect of polygonum cuspidatum and hawthorn on anti-atherosclerosis unstable plaque

作　　者：郑广娟[1,2] 朱莹[1,2] 王婷[1,2] 

机构地区：[1]广东省中医院,广东广州510120 [2]广东省中医药科学院,广东广州510006

出　　处：《中国病理生理杂志》2010年第4期635-639,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30472275);中国博士后科学基金资助项目(No.20060390123)

摘　　要：目的:观察解毒中药虎杖配伍活血中药山楂对载脂蛋白E基因敲除小鼠[ApoE(-/-)小鼠]病理形态的影响和炎症因子的变化,以探讨中药虎杖配伍山楂改善动脉粥样硬化(AS)易损斑块病变的可能调控机制。方法:将高脂饲养的ApoE(-/-)小鼠分为虎杖组、山楂组、虎杖加山楂配伍组、血脂康组、高脂饲料模型组,并设ApoE(-/-)小鼠普通饲料对照组和C57BL/6J小鼠普通饲料对照组,共分7组,每组12只小鼠。连续灌胃给药17周,取血检测炎症因子超敏C反应蛋白(hs-CRP);取主动脉,光镜观察组织结构;免疫组织化学法检测核因子-κB(NF-κB)蛋白表达。结果:高脂饲养各组小鼠及普通饲料模型组小鼠主动脉均可见不同程度的AS病理改变。各治疗组给予虎杖、山楂、虎杖加山楂配伍、血脂康后均能减轻小鼠主动脉病变情况。高脂饲料模型组血清NF-κB和hs-CRP水平均显著高于正常对照组和普通饲料组;各给药组均能明显降低NF-κB和hs-CRP表达水平,与高脂模型组相比差异显著(P<0.01),其中以虎杖加山楂配伍组降低最明显。结论:虎杖加山楂配伍可能通过降低NF-κB和hs-CRP表达,发挥抗AS的形成、发展和稳定易损斑块的作用。AIM：To investigate the effect of detoxifying herbs polygonum cuspidatum,and hawthorn,herb of promoting blood flow,on pathologic morphology and inflammatory factors in apolipoprotein E gene knockout mice,in order to approach the possible regulatory mechanism of polygonum cuspidatum and hawthorn for treating artherosclerosis （AS） unstable plaque. METHODS： The animals were divided into 7 groups （12 mice in every group）. The ApoE （-/-） mice fed with high fat diet were divided into polygonum cuspidatum group,hawthorn group,polygonum cuspidatum ＋ hawthorn group,Xuezhikang group and high fat diet model group. Moreover,ApoE （-/-） mice fed with normal diet （normal diet group） and C57BL/6J mice fed with normal diet （normal control group） were set up. After intragastric administration for 17 weeks,serum hs-CRP was detected,aorta structure was observed under light microscope and NF-κB protein expression was determined by immunohistochemistry. RESULTS： The pathological change of AS in aorta in all groups fed with high fat diet and normal diet group were observed with different degree. The changes of aortic lesion in all treatment groups were reduced. The levels of NF-κB and hs-CRP in high fat diet group were significant higher than those in normal control group and normal diet group. Serum NF-κB and hs-CRP levels decreased in every treatment group,which were significant different from those in high fat diet model group （P〈0.01）. Among them,the changes in polygonum cuspidatum and hawthorn groups were the best. CONCLUSION： Chinese herbs of polygonum cuspidatum and hawthorn reduce inflammatory factors NF-κB and hs-CRP expression and play a role in anti-AS formation.

关 键 词：虎杖配伍山楂 载脂蛋白E基因敲除小鼠 动脉粥样硬化 NF-ΚB 超敏C-反应蛋白 

分 类 号：R363[医药卫生—病理学]