葛根素对糖尿病大鼠心肌损伤的影响  

Effect of puerarin on myocardial damage in STZ-induced diabetic rats

作　　者：陈秀芳[1] 雷康福[1] 董敏[2] 方周溪[2] 金丽琴[1] 

机构地区：[1]温州医学院生物化学教研室,浙江温州325035 [2]温州医学院生物学实验教学中心,浙江温州325035

出　　处：《中国病理生理杂志》2010年第4期650-655,共6页Chinese Journal of Pathophysiology

基　　金：温州市科技局科研基金资助项目(No.Y2005A002)

摘　　要：目的:探讨葛根素对糖尿病大鼠心肌损伤的影响及其机制。方法:雄性SD大鼠随机分成6组:正常对照组,糖尿病模型组,葛根素高(160mg·kg-1)、中(120mg·kg-1)、低(80mg·kg-1)剂量治疗组,氨基胍(100mg·kg-1)治疗组;各组大鼠每天腹腔注射相应药物1次,正常对照组及糖尿病模型组腹腔注射等体积丙二醇。治疗12周后,透射电镜下观察心肌的形态学改变,用生化方法测定血糖浓度、心肌组织中超氧化物歧化酶(SOD)、Ca2+-ATPase、Na+-K+-ATPase活性及丙二醛(MDA)含量,采用RT-PCR检测心肌组织过氧化物酶体增殖物激活受体γ(PPAR-γ)、葡萄糖转运体4(GLUT-4)、醛糖还原酶(AR)的mRNA表达水平。结果:糖尿病组大鼠心肌组织电镜下主要见到心肌细胞肌原纤维减少,纤维间脂滴沉积,线粒体排列紊乱,嵴部分断裂或消失,结构不清;SOD、Ca2+-ATPase、Na+-K+-ATPase活性和PPAR-γ、GLUT-4 mRNA表达明显低于正常对照组(均P<0.01),而血糖浓度、MDA含量和AR mRNA表达明显高于正常对照组(均P<0.01)。经葛根素治疗后,上述改变逆转,与糖尿病模型组比较差异显著(P<0.05或P<0.01),电镜下心肌组织形态学病变明显减轻,肌纤维排列较整齐,仅见少量脂滴沉积,大部分线粒体嵴清晰致密。结论:葛根素对糖尿病大鼠心肌具有一定的保护作用,其机制可能与上调PPAR-γ、GLUT-4 mRNA的表达,促进心肌细胞对葡萄糖的摄取,减轻氧化应激损伤有关。AIM：To investigate the effect of puerarin on diabetic myocardial damage and to explore its possible mechanisms in rats. METHODS： The male Sprague-Dawley rats were randomly divided into normal control group,diabetic group,high dose puerarin （160 mg/kg）,middle dose puerarin （120 mg/kg）,low dose puerarin （80 mg/kg） treatment groups and aminoguanidine （100 mg/kg） treatment group. Corresponding drugs were intraperitoneally injected once a day. The animals in normal control group and diabetic group were given equal propylene glycol. 12 weeks later,the rats were sacrificed and their cardiac muscles were collected. Myocardial structure was observed under transmission electron microscope （TEM）. The blood glucose concentration and the activities of superoxide dismutase （SOD）,Ca^2＋-ATPase,Na^＋-K^＋-ATPase and the content of malondialdehyde （MDA） in myocardial homogenate were measured biochemically. In addition,the mRNA expressions of peroxisome proliferator activated receptor γ （PPAR-γ）,glucose transporter-4 （GLUT-4） and aldose reductase （AR） in myocardial tissues were determined by reverse transcription polymerase chain reaction （RT-PCR）. RESULTS： It is observed that the myofibrils were diminished,broken or fused,some lipid droplets deposited in the cytoplasm of cardiocytes and part of cristae of mitochondria were broken or disappeared under TEM in diabetic group. The activities of SOD,Ca^2＋-ATPase,Na^＋-K^＋-ATPase as well as the mRNA expressions of PPAR-γ and GLUT-4 decreased significantly （all P〈0.01）. The blood glucose concentration and the MDA level and the AR mRNA expression increased obviously （all P〈0.01） in diabetic group as compared to those in normal control group. How-ever,in puerarin treatment groups,the above changes were reversed,a significant differences of those were found as compared to those in diabetic group （P〈0.05 or P〈0.01,respectively）. The pathological change of cardiac muscles was relieved. It showed that myofibrils

关 键 词：葛根素 糖尿病 心肌 过氧化物酶体增殖物激活受体Γ 葡萄糖转运体4 氧化性应激 

分 类 号：R363[医药卫生—病理学]