黑木耳多糖对抗大鼠慢性缺血性脑损伤  

Protective effects of auricularia auricular polysaccharide on chronic cerebral ischemia injury in rats

作　　者：卢舜飞[1] 孙丽娜[2] 沈佳[2] 苏方[2] 王会平[2] 叶治国[2] 叶挺梅[1] 夏强[2] 

机构地区：[1]丽水学院医学院,浙江丽水323000 [2]浙江大学医学院生理学教研室,浙江杭州310058

出　　处：《中国病理生理杂志》2010年第4期721-724,共4页Chinese Journal of Pathophysiology

基　　金：浙江省科技厅基金资助项目(No.2006C23040)

摘　　要：目的:观察黑木耳多糖(AAP)对大鼠慢性脑缺血损伤的保护作用,并探讨其相关机制。方法:雄性成年SD大鼠右侧永久性大脑中动脉栓塞(MCAO),建立慢性脑缺血模型,缺血后每天分别给予不同浓度的AAP灌胃4周。银杏叶提取物作为阳性对照。4周后采用Morris水迷宫检测大鼠学习记忆能力。取脑做冰冻切片进行Nissl染色,观察存活神经元数量,并测定脑组织丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性。结果:AAP能明显改善脑缺血大鼠的学习记忆能力,增加海马神经元的存活数量,并且能够使脑组织长期MCAO诱导的MDA生成减少,使SOD活性显著升高。高剂量AAP(200mg/kg)的作用和银杏叶提取物相比更明显。结论:AAP明显减轻大鼠慢性脑缺血损伤,其作用与其对抗过氧化应激有关。AIM：To investigate the effects of auricularia auricular polysaccharide （AAP） on chronic cerebral ischemia injury in rats. METHODS： The chronic cerebral ischemia mode1 was made by permanent middle cerebral artery occlusion （MCAO） on the right side. AAP at different doses （50 mg/kg and 100 mg/kg） was intragastrically administered at the onset of ischemia and in the following days after operation,once a day for 4 weeks. After 4 weeks of MCAO,Morris water maze test was introduced to examine the learning and memory functions. Nissl staining was performed to detect the survival neurons in hippocampal slices. Level of malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） in brain tissue were measured. RESULTS： Rats treated with AAP showed a shorter escaping latency in spacial navigation test because the AAP treated rats spent less time to find the platform in spatial probe test. More survival neurons in hippocampal slices were observed from AAP treated rats. Also,the MDA level in brain tissue was reduced and SOD activity in brain tissue was increased in the AAP treated rats with MCAO. CONCLUSION： AAP protects rats from chronic brain ischemic injury,in which its anti-oxidative effect might be involved.

关 键 词：黑木耳多糖 脑缺血 MORRIS水迷宫 丙二醛 超氧化物歧化酶 

分 类 号：Q492[生物学—生理学]