白花丹参对大鼠脑缺血再灌注致线粒体损伤及细胞凋亡的影响  

Effects of Salvia miltiorrhiza Bunge.f.alba. on mitochondrial damage and apoptosis induced by cerebral ischemia and reperfusion

作　　者：张秋玲[1] 孙远标[2] 王海英[1] 宋述杰[3] 白波[1] 

机构地区：[1]泰山医学院生理教研室,山东泰安271000 [2]泰安市中医医院脑病康复科,山东泰安271000 [3]泰山医学院临床医学系,山东泰安271000

出　　处：《中国病理生理杂志》2010年第4期725-729,共5页Chinese Journal of Pathophysiology

基　　金：山东省科技攻关资助项目(No.2006GG2202037);山东省教育厅资助课题(No.J06L20)

摘　　要：目的:通过观察白花丹参对局灶性脑缺血再灌注损伤大鼠脑细胞线粒体超微结构、氧化应激功能及脑细胞凋亡的影响,探讨白花丹参对抗脑缺血再灌注损伤的作用及其机制。方法:采用大脑中动脉内线栓阻断法(MCAO)造成局灶性脑缺血再灌注模型,将SD大鼠随机分为:假手术组、单纯脑缺血再灌注组、白花丹参组及丁苯酞治疗对照组,按照6.5g/kg BW白花丹参生药及15mg丁苯酞的剂量灌胃给药,每天1次,分别于再灌注后12h、24h及48h取损伤侧大脑,应用电镜观察脑细胞超微结构的改变、比色法检测脑组织线粒体丙二醛(MDA)含量及谷胱甘肽过氧化物酶(GSH-Px)活性的变化、流式细胞技术检测脑细胞凋亡率的变化。结果:脑缺血再灌注后大鼠脑细胞内出现大量高电子密度中毒颗粒及空泡,线粒体膜崩解、内嵴消失;MDA含量明显增高(P<0.05);GSH-Px酶活性显著降低(P<0.05);脑细胞大量凋亡。与脑缺血再灌注组比较,白花丹参能够减少细胞内高电子密度中毒颗粒及空泡,增强线粒体膜的稳定性,显著降低MDA含量(P<0.05),延缓GSH-Px酶活性的下降并保护其活性(P<0.05),显著降低脑细胞凋亡率(再灌注后24h及48h组,P<0.05)。结论:白花丹参可减轻大鼠脑缺血再灌注所致线粒体损伤、延缓GSH-Px酶活性下降,抑制细胞凋亡,从而发挥脑保护作用。AIM：To observe the effects of Salvia miltiorrhiza Bunge.f.alba. （Sal） on the mitochondrial ultra-structure,oxidative stress and apoptosis induced by ischemia injury in a rat model of focal cerebral ischemia and reperfusion.METHODS： The middle cerebral artery occlusion/reperfusion （MCAO/R） rat model was established by a modified Longa occlusion method. Adult male SD rats were randomly divided into control group,simple ischemia reperfusion group,Sal with ischemia reperfusion group and butylphthalide with ischemia reperfusion group. To study the protective effects of Sal and its mechanism,the intervention of Sal was given and the ultra-structure of mitochondria,functions of mitochondria under oxidative stress and the incidence of apoptosis of brain cells were determined.RESULTS： Many electron dense toxic granulation and vacuolus in mitochondria were observed in the rat brain of focal cerebral ischemia and reperfusion. Under the condition of ischemia and reperfusion,the mitochondria membrane was disaggregative,and the tubular cristae of mitochondrion disappeared. MDA content was obviously increased and the activity of glutathione peroxidase decreased significantly. The apoptosis of brain cells were observed in a great quantity. The changes of ultra-structure of mitochondria and the activity of GSH-Pxase were significantly improved by the treatment of Sal. Furthermore,treatment with Sal delayed the decrease of GSH-Pxase activity,and inhibited the increase in MDA content in brain tissue after ischemia and reperfusion. The incidence of apoptosis of brain cells was also decreased.CONCLUSION： Sal protects the brain tissue from ischemia injury.

关 键 词：脑缺血 再灌注损伤 细胞凋亡 白花丹参 

分 类 号：R743[医药卫生—神经病学与精神病学]