BMP_2活性多肽/鼠尾Ⅰ型胶原复合物植入异位成骨的实验研究  

Experimental Study on Ectopic Osteogenesis of a BMP_2-derived Peptide/Type Ⅰ Collagen Compound

作　　者：李景峰[1] 郑启新[1] 郭晓东[1] 卢宏伟[1] 林振宇[1] 兰生辉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022

出　　处：《中国生物医学工程学报》2010年第2期295-299,共5页Chinese Journal of Biomedical Engineering

基　　金："十一五"国家高技术研究发展计划(863)干细胞与组织工程重大项目(2006AA 02A124);国家自然科学基金资助项目(30470483);教育部"新世纪优秀人才支持计划资助"(NCET-05-0647)

摘　　要：用动物实验的方法验证鼠尾Ⅰ型胶原支架与自行研制合成的BMP2活性多肽复合后诱导异位成骨的能力与作用。自行提取鼠尾Ⅰ型胶原以及与BMP2活性多肽复合,冻干后低真空模式下电镜观察胶原结构。实验分2组。对照组:单纯鼠尾Ⅰ型胶原组;实验组:BMP2活性多肽/鼠尾Ⅰ型胶原复合物组。将12只大白鼠随机分成2组,每只大白鼠右侧大腿作2 cm的切口,制备股四头肌肌袋模型,将上述材料分别植入肌袋。术后第3周和第6周分别作放射学检查(X-ray,CT),第6周将所有大白鼠处死作组织学(HE染色)检查。鼠尾Ⅰ型胶原冻干后孔径大小合适与BMP2活性多肽复合后无明显改变。术后第3周和第6周放射学检查可见实验组有明显的钙化影形成,且第6周成骨范围要明显大于第3周时所见,而对照组无成骨现象。术后第6周组织学观察可见实验组植入区有成骨细胞和大量新骨形成,而对照组仅见炎性细胞改变。鼠尾Ⅰ型胶原是一种较好的载体支架材料,自行研制合成的BMP2活性多肽与其复合后,具有较强的异位诱导成骨能力。The aim of this study is to investigate the ectopic osteogenetic capacity of synthesis BMP2-derived peptide/type Ⅰ collagen from rat rail compound by animal experiment. We extracted type I collagen from rat rail by oneself and then observed its structure after freeze-dried under low-vacuum electron microscopy. Twelve SD rats were divided into two groups with 6 in each group. Pure type Ⅰ collagen from rat rail was implanted as control group,while the BMP2-derived peptide/type Ⅰ collagen from rat rail complex was implanted as experiment group. The model of quadriceps femoris myo-bag was established by implanting the above material in the myo-bag on the right side thigh of each rat with 2 cm opening,separately. The rats were made the radioactivity inspection （X-ray,CT） separately at the 3rd and 6th week after implantation. At the 6thweek,all rats were killed and tissue response was observed histologically （ HE）. The diameter of the pores of type Ⅰ collagen from rat rail after freeze drying was appropriate,and that of the BMP2-derived peptide/type Ⅰ collagen from rat rail compound did not change obviously. At the 3rd and 6th week after implantation,radioactivity inspection showed that experimental group had the obvious calcification shade formation. At the 6th week,the scope became bigger than that of the 3rd week,but the control group was not the osteogenesis phenomenon. At the 6th week,histology observation indicated that experimental group had osteoblasts and massive new bone formation around the implants,but the control group was only found inflammatory cells. Type Ⅰ collagen from rat rail was a better scaffold material for bone tissue engineering. BMP2-derived peptide complexed with rat rail collagen could induce ectopic osteogenesis more effectively.

关 键 词：BMP2活性多肽 鼠尾胶原 异位成骨 

分 类 号：R318[医药卫生—生物医学工程]