人脐带沃顿胶间充质干细胞对人外周血T淋巴细胞的免疫调节作用  

Immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells from human umbilical cord on human peripheral blood T lymphocytes

作　　者：周长辉[1,2] 田毅[2,3] 杨波[2,3] 胡祥[4,5] 焦红亮 周云帆[1] 王成春[1] 谷晨熙[6] 雷宁静[1] 关方霞[1] 

机构地区：[1]郑州大学生物工程系,河南省郑州市450001 [2]河南省高等学校临床医学重点学科开放实验室,河南省郑州市450052 [3]郑州大学第一附属医院神经外科,河南省郑州市450052 [4]江苏省干细胞与生物治疗公共技术服务平台,江苏省泰州市225300 [5]深圳市北科细胞工程研究所,广东省深圳市518000 [6]郑州大学临床医学系,河南省郑州市450001

出　　处：《中国组织工程研究与临床康复》2010年第14期2485-2491,共7页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：郑州大学211三期建设项目"干细胞基础与临床研究";河南省科技厅09科技发展计划(092102310250);郑州市二七区科技局社会发展项目(20095157);江苏省干细胞与生物治疗公共技术服务平台(BM2008146)~~

摘　　要：背景:骨髓源性间充质干细胞具有低免疫原性和免疫调节作用,但有关人脐带沃顿胶间充质干细胞的免疫调节作用及其机制研究较少。目的:观察脐带沃顿胶间充质干细胞对异体外周血T淋巴细胞的免疫调节作用及机制。方法:从人脐带沃顿胶组织块培养中分离出间充质干细胞。将脐带沃顿胶间充质干细胞及脐带沃顿胶间充质干细胞培养上清分别与植物凝集素刺激下的人外周血T淋巴细胞共培养,多功能酶标仪测量脐带沃顿胶间充质干细胞及脐带沃顿胶间充质干细胞培养上清与植物凝集素刺激下的人外周血T淋巴细胞共培养72h后A值;ELISA试剂盒检测培养上清中转化生长因子β1、γ-干扰素的表达。结果与结论:沃顿胶间充质干细胞对植物凝集素刺激下的T淋巴细胞增殖有抑制作用,增殖抑制率为56%(P<0.01),沃顿胶间充质干细胞培养上清对植物凝集素刺激下的T淋巴细胞增殖也具有抑制作用,且这种抑制呈剂量依赖性。含50%沃顿胶间充质干细胞培养上清组和100%培养上清组的增殖抑制率分别为8.3%、27%(P<0.05);沃顿胶间充质干细胞能显著降低T淋巴细胞分泌的γ-干扰素(P<0.05);可分泌转化生长因子β1,沃顿胶间充质干细胞与T淋巴细胞共培养组的转化生长因子β1分泌量低于沃顿胶间充质干细胞单独培养组(P<0.05)。提示沃顿胶间充质干细胞及沃顿胶间充质干细胞培养上清对植物凝集素刺激下的T淋巴细胞增殖均有抑制作用,其作用机制可能与细胞间的接触及抑制T淋巴细胞分泌的γ-干扰素有关。BACKGROUND：Bone marrow mesenchymal stem cells have low immunogenicity and immunomodulatory effect,but there are seldom reports concerning the immunomodulatory effect of Wharton＇s jelly-derived mesenchymal stem cells of human umbilical cord and its mechanims.OBJECTIVE：To investigate the immunomodulatory effects and mechanisms of Wharton＇s jelly-derived mesenchymal stem cells of human umbilical cord on varient peripheral blood T lymphocytes.METHODS：Mesenchymal stem cells were isolateded from Wharton＇s jelly of human umbilical cord by tissue culture.T lymphocytes from human peripheral blood were stimulated by phytohemagglutinin and co-cultured with umbilical cord Wharton＇s jelly-derived mesenchymal stem cells and umbilical cord Wharton＇s jelly-derived mesenchymal stem cells supernatant respectively to measure A value following 72 hours of coculture using multifunctional microplate reader.Expression of cytokines including transforming growth factor-beta 1（TGF-β1） and interferon-γ（IFN-γ） was evaluated by enzyme-labeled immunosorbent assay.RESULTS AND CONCLUSION：Wharton＇s jelly-derived mesenchymal stem cells could inhibite the proliferation of T lymphocytes induced by phytohemagglutinin.The proliferation inhibition rate was 56%（P〈 0.01）.Wharton＇s jelly-derived mesenchymal stem cells supernatant also had inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin,in a dose-dependent fashion.The proliferation inhibition rates were 8.3% and 27% respectively in the 50% Wharton＇s jelly-derived mesenchymal stem cells supernatant and 100% Wharton＇s jelly-derived mesenchymal stem cells supernatant groups（P〈 0.05）.Wharton＇s jelly-derived mesenchymal stem cells significantly decreased γ-interferon secrted from T-lymphocytes（P 0.05）.The secretion of TGF-β1 was lower in the coculture of Wharton＇s jelly-derived mesenchymal stem cells and T lymphocytes group than Wharton＇s jelly-derived mesenchymal stem cells alone group（P 〈0.05）.These indicate

关 键 词：沃顿胶间充质干细胞 T淋巴细胞 免疫调节 Γ-干扰素 转化生长因子Β1 

分 类 号：R734.2[医药卫生—肿瘤]