人永生化细胞系模型建立的方法  

Methods of establishing human immortalized cell lines

作　　者：汪廷乐[1] 宋萌[1] 

机构地区：[1]上海交通大学附属第一人民医院口腔科,上海200080

出　　处：《上海口腔医学》2010年第2期212-215,共4页Shanghai Journal of Stomatology

基　　金：上海市科学技术委员会资助(重点)项目(08JC1412900);上海市重点学科项目(S30206-kf05)~~

摘　　要：肿瘤的发生是一个多基因、多阶段的复杂过程,其中致癌基因与抑癌基因发挥了重要作用。目前对肿瘤的发生机制仍不十分清楚,无法在体内研究肿瘤发生的全过程,为此需要借助于体外细胞模型来完成该类研究。目前,绝大多数研究只是基于啮齿动物细胞系水平,但要明确致癌物质的确切作用,则需要人细胞模型。因此,建立永生化人细胞系就显得十分重要。本文对人永生化细胞系模型建立的各种方法进行了讨论。The development of cancer is a multistages process that involving widely varies genes. The oncogenes and anti-oncogenes play an important role during this process. However,little is known about the molecular mechanisms responsible for the development. We cann＇t understand the process of cancer in human bodies actually,so we should rely on the use of the human cells in culture. While the majority of carcinogenesis studies have relied on the use of rodent cells in culture,experimental models to define the role of carcinogenic agents in the development of cancer must be established by using a variety of human cells.Therefore,a variety of human cell lines need to be established.

关 键 词：永生化 猿猴病毒40 人乳头瘤病毒 人端粒酶反转录酶 

分 类 号：R730.2[医药卫生—肿瘤]